RNA extraction A+ 레포트

소개

"RNA extraction A+ 레포트"에 대한 내용입니다.

목차

1. Title
2. Name
3. Purpose
4. Materials
5. Methods
6. Results
7. Discussion
8. References

본문내용

1. Add 1㎖ of Tri-RNA reagent to 100mg tissue.
2. Homogenize tissue samples in Tri-RNA reagent by vortexing.
3. Leave the homogenates for 10 minutes at room temperature.
4. Add 0.2㎖ of chloroform and mix by vortexing. Incubation for 5 minutes (RT).
5. Centrifuge at 13,000rpm for 15 minutes to separate the phases, RNA is in the clear upper aqueous phase.
6. Transfer the 500㎕ of RNA phase to a clean tube.
7. RNA is precipitated by adding 1X volume (500㎕) of isopropanol, vortex, leave at room temperature for 10 minutes, and then centrifuge at 13,000rpm for 15 minutes.

참고자료

· 강해묵, 『생명 생물의 과학 9판 상권』, 라이프사이언스(2016), 61-64p.
· 이병무, 『유전자 클로닝과 DNA 분석』, 월드사이언스(2017), 34-38p.
· 이종호, 『의생명과학 실험서 상권』, 라이프사이언스(2021), 45-49p.
· “리보솜 RNA", 생화학백과, 생화학분자생물학회
· “Chloroform", 화학백과, 대한화학회
· “RNA", 분자·세포생물학백과, 한국분자·세포생물학회
· “RNase", 화학대사전, 화학대사전(2001)