Adherent Cell lysis for Protein extraction A+레포트입니다.

소개

Adherent Cell lysis for Protein extraction A+레포트입니다.(1). Thermo Scientific™ - Traditional Methods of Cell Lysis for Protein Extraction
https://www.thermofisher.com/kr/ko/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/traditional-methods-cell-lysis.html
(2). Martin NC, 『The use of phosphate buffered saline for the recovery of cells and spermatozoa from swabs』, Sci Justice. 2006 Jul-Sep;46(3):179-84.
https://pubmed.ncbi.nlm.nih.gov/17388245/
(3). Thermo Scientific™ - RIPA Lysis and Extraction Buffer
https://www.thermofisher.com/order/catalog/product/kr/ko/89900
(4). Shehadul Islam M, Aryasomayajula A, Selvaganapathy PR. 『A Review on Macroscale and Microscale Cell Lysis Methods』, Micromachines (Basel). 2017 Mar 8;8(3):83,
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190294/
(5). Labome - Protein Quantitation
https://www.labome.com/method/Protein-Quantitation.html

목차

1. Title
2. Date
3. Name
4. Purpose
5. Materials
6. Methods
7. Results
8. Discussions
9. Project
10. Reference

본문내용

1. Title
Adherent Cell lysis for Protein extraction

2. Date

3. Name

4. Purpose
Cell lysis (or cellular disruption)에 사용되는 여러 가지 물리적, 화학적 방법을 알아본 후, RIPA lysis buffer를 사용하여 세포를 파괴하고 cellular protein을 얻을 수 있다.

5. Materials
MCF-7 Cells (cultured in 60㎜ dish), Ice cold DPBS, Ice cold RIPA lysis buffer, micropipette, tip, rack, beaker, cell scraper, ep-tube, centrifuge, ice, tissue, 70% ethanol

6. Methods
① Micropipette으로 media를 제거한다.

참고자료

· Thermo Scientific™ - Traditional Methods of Cell Lysis for Protein Extraction
· https://www.thermofisher.com/kr/ko/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/traditional-methods-cell-lysis.html
· Martin NC, 『The use of phosphate buffered saline for the recovery of cells and spermatozoa from swabs』, Sci Justice. 2006 Jul-Sep;46(3):179-84.
· https://pubmed.ncbi.nlm.nih.gov/17388245/
· Thermo Scientific™ - RIPA Lysis and Extraction Buffer
· https://www.thermofisher.com/order/catalog/product/kr/ko/89900
· Shehadul Islam M, Aryasomayajula A, Selvaganapathy PR. 『A Review on Macroscale and Microscale Cell Lysis Methods』, Micromachines (Basel). 2017 Mar 8;8(3):83,
· https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190294/
· (5). Labome - Protein Quantitation
· https://www.labome.com/method/Protein-Quantitation.html