• AI글쓰기 2.1 업데이트
SILVER
SILVER 등급의 판매자 자료
리포트 / 공학/기술

[생물공정실험] 5주차 Spore sterilization for food processing 예비보고서

"[생물공정실험] 5주차 Spore sterilization for food processing 예비보고서"에 대한 내용입니다.
4 페이지
어도비 PDF
최초등록일 2023.01.15 최종저작일 2022.12
아주대 생물공정 생물공정실험 실험
파트너스 간편링크 파트너스
보고계신 자료로 파트너스
홍보 수익을 창출해보세요.
4P 미리보기
[생물공정실험] 5주차 Spore sterilization for food processing 예비보고서
상세정보 자료후기 0 자주묻는질문

  • 미리보기

    목차

    1. 실험목적
    2. 실험이론
    3. 실험방법
    4. 참고문헌

    본문내용

    (1) agar plate에서 colony 1개를 고른다. 이 박테리아 샘플을 3ml의 BHI broth에서 37℃로 12시간 배양한다.
    (2) PBS를 사용하여 OD600 0.5까지 희석합니다. 배양액 250 μl를 70:30 한천 판에 펴 바르고 37 ℃에서 3일간 혐기적으로 배양한다.
    (3) 배양 후 5ml PBS를 사용하여 스프레더로 플레이트에서 검체를 채취합니다.
    (4) 13000rpm으로 셀을 실온에서 5분간 원심분리하고 상등액을 제거합니다. 5ml PBS에서 셀을 다시 일시 중단합니다.
    (5) 95% EtOH 5ml를 세포 혼합물에 넣고 소용돌이를 잘 치고 RT에서 1시간 동안 배양합니다.
    (6) 셀을 13000rpm으로 5분간 원심분리하고 상등액을 제거합니다. 10ml PBS에서 셀을 다시 일시 중단합니다.
    (7) 포자 스톡을 70℃까지 20분간 가열한다. 포자 스톡이 RT로 냉각되도록 합니다.

    참고자료

    · Physical and Chemical Method of Sterilization, Sterile Safequip & Chemicals LLP.
    · 식품공학 chapter 5. Preservation Process
    · Ⅲ. 우유 열처리에 의한 병원균 사멸효과, koreascience

  • AI와 토픽 톺아보기

    • 1. Spore sterilization for food processing
      Spore sterilization is a critical process in food processing to ensure the safety and quality of food products. Spores, which are the dormant, resistant forms of certain bacteria, can survive many conventional sterilization methods and pose a significant risk of contamination if not properly eliminated. Effective spore sterilization is particularly important for low-acid foods, where the presence of spores can lead to the growth of harmful bacteria like Clostridium botulinum, which can cause botulism, a severe and potentially fatal form of food poisoning. The choice of spore sterilization method depends on the specific food product, its composition, and the target microorganisms. Common spore sterilization techniques include heat treatment, irradiation, and the use of chemical agents. Each method has its own advantages and disadvantages in terms of effectiveness, cost, and impact on the sensory and nutritional properties of the food. Careful validation and monitoring of the spore sterilization process is crucial to ensure its efficacy and consistency. Regulatory agencies like the FDA and USDA have established guidelines and standards for spore sterilization in food processing to protect public health. Ongoing research and innovation in this field are essential to develop more efficient and sustainable spore sterilization technologies that can meet the evolving needs of the food industry.
    • 2. Pasteurization
      Pasteurization is a critical food processing technique that involves the controlled application of heat to kill pathogenic microorganisms and extend the shelf life of food products. This process is particularly important for dairy products, juices, and other perishable foods that can harbor harmful bacteria like Listeria, Salmonella, and E. coli. The primary goal of pasteurization is to reduce the number of viable pathogens to a level that is safe for human consumption, without significantly altering the sensory and nutritional properties of the food. The specific time and temperature parameters of pasteurization vary depending on the food product, the target microorganisms, and the desired level of microbial reduction. Effective pasteurization can significantly improve food safety and public health by reducing the risk of foodborne illnesses. It is a well-established and widely adopted technology in the food industry, with a long history of successful implementation. However, it is important to note that pasteurization does not completely eliminate all microorganisms, and proper handling and storage of pasteurized products are still necessary to maintain food safety. Ongoing research and innovation in pasteurization techniques, such as the development of novel heat treatment methods and the integration of advanced monitoring and control systems, can further enhance the efficiency and effectiveness of this critical food processing technology.
    • 3. Sterilization
      Sterilization is a fundamental process in various industries, including healthcare, food processing, and pharmaceutical manufacturing, to eliminate all forms of microbial life, including bacteria, viruses, fungi, and spores. Effective sterilization is crucial for ensuring the safety and quality of products, preventing the spread of infectious diseases, and protecting public health. There are several sterilization methods available, each with its own advantages and limitations. These include heat-based sterilization (e.g., autoclaving, dry heat), chemical sterilization (e.g., ethylene oxide, hydrogen peroxide), and physical sterilization (e.g., ionizing radiation, filtration). The choice of sterilization method depends on the nature of the product or material being sterilized, the target microorganisms, and the desired level of microbial reduction. Proper validation and monitoring of the sterilization process are essential to ensure its efficacy and consistency. Regulatory agencies, such as the FDA and ISO, have established guidelines and standards for sterilization in various industries to ensure the safety and quality of products. Ongoing research and innovation in sterilization technologies, such as the development of more efficient and environmentally friendly methods, can help to improve the effectiveness, sustainability, and accessibility of sterilization processes. As the demand for safe and sterile products continues to grow, the importance of sterilization will only become more critical in the years to come.
    • 4. Heat sterilization
      Heat sterilization is a widely used and highly effective method for eliminating microorganisms, including bacteria, viruses, and spores, from various products and materials. This process involves the application of heat, either through moist heat (e.g., autoclaving) or dry heat (e.g., hot air ovens), to destroy the cellular structures and vital functions of microorganisms. The effectiveness of heat sterilization depends on factors such as the temperature, duration of exposure, and the specific characteristics of the target microorganisms. Moist heat sterilization, such as autoclaving, is generally more effective than dry heat sterilization, as the presence of water molecules can enhance the penetration and disruption of microbial cells. Heat sterilization is commonly used in healthcare settings to sterilize medical equipment, surgical instruments, and other critical items. It is also widely employed in the food and pharmaceutical industries to ensure the safety and quality of products. The process can be tailored to different materials and products, making it a versatile and adaptable sterilization method. While heat sterilization is highly effective, it is important to carefully monitor and validate the process to ensure consistent and reliable results. Improper application of heat can lead to incomplete sterilization or damage to the product or material being sterilized. Ongoing research and innovation in heat sterilization technologies, such as the development of more energy-efficient and environmentally friendly methods, can help to further improve the efficiency and sustainability of this critical process.
    • 5. Filtration sterilization
      Filtration sterilization is a physical method of removing microorganisms from liquids, gases, or other fluids by passing them through a filter with pores small enough to trap and retain the target microorganisms. This process is particularly useful for sterilizing heat-sensitive materials, such as certain pharmaceutical and biological products, where heat-based sterilization methods may not be suitable. The effectiveness of filtration sterilization depends on the size and characteristics of the filter, the properties of the fluid being filtered, and the specific microorganisms that need to be removed. Commonly used filtration methods include membrane filtration, depth filtration, and tangential flow filtration, each with its own advantages and limitations. Filtration sterilization is widely used in various industries, including pharmaceuticals, biotechnology, and food processing, to ensure the safety and purity of critical products and materials. It is often used as a final step in the manufacturing process to remove any remaining microbial contaminants. One of the key advantages of filtration sterilization is its ability to remove a wide range of microorganisms, including bacteria, viruses, and even some prions, without the need for heat or chemical treatment. This makes it a valuable tool for preserving the integrity and functionality of sensitive materials. However, filtration sterilization is not without its challenges. Factors such as filter clogging, fouling, and the potential for microbial breakthrough can affect the reliability and consistency of the process. Ongoing research and development in filtration technologies, including the use of novel membrane materials and advanced monitoring systems, aim to address these challenges and further improve the efficiency and effectiveness of filtration sterilization.

  • 자료후기

      Ai 리뷰
      이 문서는 포자 살균 기술에 대한 전반적인 이해를 제공하며, 실험을 통해 각 방법의 장단점을 구체적으로 확인할 수 있습니다.

    • 자주묻는질문의 답변을 확인해 주세요

      해피캠퍼스 FAQ 더보기

      꼭 알아주세요

      • 자료의 정보 및 내용의 진실성에 대하여 해피캠퍼스는 보증하지 않으며, 해당 정보 및 게시물 저작권과 기타 법적 책임은 자료 등록자에게 있습니다.
        자료 및 게시물 내용의 불법적 이용, 무단 전재∙배포는 금지되어 있습니다.
        저작권침해, 명예훼손 등 분쟁 요소 발견 시 고객센터의 저작권침해 신고센터를 이용해 주시기 바랍니다.
      • 해피캠퍼스는 구매자와 판매자 모두가 만족하는 서비스가 되도록 노력하고 있으며, 아래의 4가지 자료환불 조건을 꼭 확인해주시기 바랍니다.
        파일오류 중복자료 저작권 없음 설명과 실제 내용 불일치
        파일의 다운로드가 제대로 되지 않거나 파일형식에 맞는 프로그램으로 정상 작동하지 않는 경우 다른 자료와 70% 이상 내용이 일치하는 경우 (중복임을 확인할 수 있는 근거 필요함) 인터넷의 다른 사이트, 연구기관, 학교, 서적 등의 자료를 도용한 경우 자료의 설명과 실제 자료의 내용이 일치하지 않는 경우
    • 정가
      2,500원
      판매자스토어판매자스토어 자료문의자료문의 링크복사 링크복사
      • 지식판매자
        노루궁뎅이 S
      • 페이지
        4 페이지 어도비 PDF
      • 최초등록일
        2023.01.15
      • 최종저작일
        2022.12
    문서 초안을 생성해주는 EasyAI
    안녕하세요 해피캠퍼스의 20년의 운영 노하우를 이용하여 당신만의 초안을 만들어주는 EasyAI 입니다.
    저는 아래와 같이 작업을 도와드립니다.
    - 주제만 입력하면 AI가 방대한 정보를 재가공하여, 최적의 목차와 내용을 자동으로 만들어 드립니다.
    - 장문의 콘텐츠를 쉽고 빠르게 작성해 드립니다.
    - 스토어에서 무료 이용권를 계정별로 1회 발급 받을 수 있습니다. 지금 바로 체험해 보세요!
    이런 주제들을 입력해 보세요.
    - 유아에게 적합한 문학작품의 기준과 특성
    - 한국인의 가치관 중에서 정신적 가치관을 이루는 것들을 문화적 문법으로 정리하고, 현대한국사회에서 일어나는 사건과 사고를 비교하여 자신의 의견으로 기술하세요
    - 작별인사 독후감
    “EasyAI 가 작성한 문서 초안은 완벽하지 않을 수 있습니다"
    EasyAI 이동하기
    해캠 AI 챗봇과 대화하기
    챗봇으로 간편하게 상담해보세요.
    2026년 01월 18일 일요일
    AI 챗봇
    안녕하세요. 해피캠퍼스 AI 챗봇입니다. 무엇이 궁금하신가요?
    7:02 오전