Lewin's Essential GENES 분자생물학 4판 정리노트 03. 분단된 유전자

목차

1. Introduction
2. An Interrupted Gene Consists of Exons and Introns
3. Organization of Interrupted Genes May Be Conserved
4. Exon Sequences Are Conserved but Introns Vary
5. Genes Show a Wide Distribution of Sizes Primarily Due to Intron Size and Number Variation
6. Some DNA Sequences Code for More Than One Polypeptide
7. Some Exons Can Be Equated with Protein Functions
8. The members of a gene family have a common organization

본문내용

3.1 Introduction
Exon: 단백질 암호화 부위(coding region)
Intron (intervening sequence) : noncoding region, Exon 사이에 존재하고 Splicing에 의해 제거됨
RNA splicing: primary transcript/pre-mRNA에서 intron 제거-> mRNA에는 coding region만 있음

3.2 An Interrupted Gene Consists of Exons and Introns
Introns are removed by the process of RNA splicing
Only mutations in exons can affect polypeptide sequence

3.3 Organization of Interrupted Genes May Be Conserved
Introns can be detected by the presence of additional regions

분단되지 않은 유전자의 mRNA를 유전자의 RNA와 hybridization
intron은 mRNA와 hybridization되지 않고 loop가 형성

cDNA(mRNA)와 genomic DNA의 restriction map을 비교할 때, cDNA에서는 introns에서 유래하는 fragments가 나타나지 않음

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