SDS-PAGE 및 Staining(A+보장, 출처가 확실한 양질의 다양한 자료, 검토를 거쳐 더욱 완벽해진 내용)

소개

"SDS-PAGE 및 Staining(A+보장, 출처가 확실한 양질의 다양한 자료, 검토를 거쳐 더욱 완벽해진 내용)"에 대한 내용입니다.

목차

1. Subject

2. Date

3. Purpose

4. Introduction
1) SDS-PAGE 실험 시 사용되는 재료의 명칭 (Bio-rad 제품)
2) SDS-PAGE의 원리
3) Staining & Destaining 원리
4) 실험에서 사용되는 시약의 조성 및 역할

5. Material & Method
1) SDS-PAGE

6. Result
1) SDS-PAGE
2) Staining & Destaining
3) E.coli streaking, spreading

7. Discussion

8. Reference

9. Assignment
1) E.coli streaking, spreading의 정의 및 목적

본문내용

1. Subject - SDS-PAGE 및 Staining

<중 략>

4. Introduction
1.SDS-PAGE 실험 시 사용되는 재료의 명칭 (Bio-rad 제품)
※ 사용 시 주의 사항 및 자세한 사용법은 ‘10) 실험’ 항목에서 후술.

1) Glass plate : Long & Short plate로 구성. Spacer plate의 경우 볼록하게 튀어나온 면이 있음. Short plate 와 Spacer plate를 결합하여 Polyacrylamide gel을 주조하기위해 사용.
2) Casting frame : 틈에 Glass plate을 끼워 넣어 사용. Glass plate를 고정 시키는 기능.
3) Casting stand : Glass plate이 장착 된 Casting frame을 고정시켜 gel을 굳히는 기능.
4) Casting Stand Gaskets : Casting stand과 함께 사용. 고무재질로 Casting frame의 밑바닥을 밀봉시킴.
5) Comb : gel을 굳힐 때 sample이 들어갈 well을 만드는 기능.

참고자료

· Jeremy M. Berg, 2007, Biochemistry 6th ed, W.H.Freeman&Company
· “SDS-PAGE”, wikipedia, 2020.10.01., https://en.wikipedia.org/wiki/SDS-PAGE
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