Differential Expression of TPX2 upon Differentiation of Human Embryonic Stem Cells

서지정보

· 발행기관 : 한국동물번식학회
· 수록지 정보 : Reproductive & developmental biology / 31권 / 4호
· 저자명 : Hye Min Noh, Seong-Jun Choi, Sehee Kim, Kye-Seong Kim, Jin Kyeoung Kim

목차

ABSTRACT INTRODUCTION MATERIALS AND METHODS Culture of Human ES Cells Differentiation of Human ES Cells into Neural Precursor Cells Immunocytochemical Analysis Extraction of RNA Synthesis of cDNA by Reverse Transcription Suppression Subtractive Hybridization Analysis of Gene Expression by Semi-Quantitative RT-PCR RESULTS Identification of Differentially Expressed Genes by SSH Un-subtracted Down-Regulation of TPX2 Expression during Neural Differentiation DISCUSSION REFFRENCES

영어초록

Embryonic stem (ES) cells are known to have an infinite proliferation and pluripotency that are associated with complex processes. The objective of this study was to examine expression of genes differentially regulated during differentiation of human ES cells by suppression subtractive hybridization (SSH). Human ES cells were induced to differentiate into neural precursor cells via embryoid body. Neural precursor cells were isolated physically based on morphological criteria. Immunocytochemical analysis showed expression of pax6 in neural precursor cells, confirming that the isolated cells were neural precursor cells. Undifferentiated human ES cells and neural precursor cells were subject to the SSH. TPX2 (Targeting Protein for Xklp2 (Xenopus centrosomal kinesin-like protein 2)) was identified, cloned and analyzed during differentiation of human ES cells into neural lineages. Expression of TPX2 was gradually down-regulated in embryoid bodies and neural precursor cells relative to undifferentiated ES cells. Targeting Protein for Xklp2 has been shown to be involved in cell division by interaction with microtubule development in cancer cells. Taken together, result of this study suggests that TPX2 may be involved in proliferation and differentiation of human ES cells.

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