Viability of Somatic Cell Nuclear Transfer Embryos following Embryo Transfer in Korean Native Striped Cattle(Bos Namadicus Falconer, Chikso)
(주)코리아스칼라
- 최초 등록일
- 2016.04.01
- 최종 저작일
- 2007.12
- 6페이지/ 어도비 PDF
- 가격 4,000원
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 31권 / 4호
ㆍ저자명 : Dae-jin Kwon, Joo-hee Park, Hwan-Sub Hwang, Yeon-Soo Park, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Oocyte Collection and In Vitro Maturation (IVM) In Vitro Fertilization (IVF) Nuclear Transfer Vutrufucatuib if Embryos Preparation of Recipient Cows Embryo Transfer and Pregnancy Diagnosis Microsatellite Assay Statistical Analysis RESULTS DISCUSSION ACJBIWKEDGEMENT REFERENCES
영어 초록
This study was conducted to examine the viability of Korean native striped cattle (Bos namadicus Falconer, Chikso) clone embryos after embryo transfer. Chikso somatic cell nuclear transfer (SCNT) embryos were produced by fusion of ear skin cells derived from a female Chikso with enucleated oocytes matured in vitro for 18-24 hr. After in vitro culture of SCNT embryos for 7 to 8 days, fresh or vitrified blastocysts derived from SCNT were transferred into a uterine horn of recipient cows. Fifteen of total 43 recipients were pregnant at Day 50 and 4 recipients were maintained to term. Three IVF-derived calves and 1 clone Chikso calf were born. Pregnancy rate was higher when fresh embryos were transferred to recipients compared to vitrified embryos, but development to term was not different between both groups. The clone Chikso calf died at 5 days after birth due to the fullness of amniotic fluid in rumen and the infection of umbilical cord. The result of the present study shows that clone Chikso calf can produced from the embryo transfer of SCNT embryos, however, solution of abortion problem is necessary to improve the cloning efficiency.
참고 자료
없음
"Reproductive & developmental biology"의 다른 논문
더보기 (4/9)