Comparative Study of Protein Profile during Development of Mouse Placenta

서지정보

· 발행기관 : 한국동물번식학회
· 수록지 정보 : Reproductive & developmental biology / 31권 / 4호
· 저자명 : Rong Xun Han, Hong Rye Kim, Kenji Naruse, Su Min Choi, Baek-Chul Kim, Chang Sik Park, Dong Il Jin

목차

ABSTRACT INTRODUCTION MATERIALS AND METHODS Placental Samples Extraction of Solubilized Proteins from Placenta 2-D Gel Electrophoresis Staining and Image Analysis of 2-D-Gels Sample Preparation for MALDI-TOF Mass Spectro-metry Analysis RESULTS Analysis of Placenta Proteomes by 2-DE Identification of Differentially Expressed Spots DISCUSSION REFERENCES

영어초록

To examine the differential protein expression pattern in the 11.5 day post-coitus (dpc) and 18.5 dpc placenta of mouse, we have used the global proteomics approach by 2-D gel electrophoresis (2-DE) and MALDI-TOF-MS. The differential protein patterns of 3 placentae at the 11.5 dpc and 18.5 dpc from nature mating mice were analyzed. Proteins within isoelectric point range of 3.0~10.0, separately were analyzed in 2DE with 3 replications of each sample. A total of approximately 1,600 spots were detected in placental 2-D gel stained with Coomassie-blue. In the comparison of 11.5 dpc and 18.5 dpc placentae, a total of 108 spots were identified as differentially expressed proteins, of which 51 spots were up-regulated proteins such as alpha-fetoprotein, mKIAA0635 protein and transferrin, annexin A5, while 48 spots were down-regulated proteins such as Pre-B-cell colony-enhancing factor 1(PBEF), aldolase 1, A isoform, while 4 spots were 11.5 dpc specific proteins such as chaperonin and Acidic ribosomal phosphoprotein P0, while 3 spots were 18.5 dpc specific proteins such as aldo-keto reductase family 1, member B7 and CAST1/ERC2 splicing variant-1. Most identified proteins in this analysis appeared to be related with catabolism, cell growth, metabolism and regulation. Our results revealed composite profiles of key proteins involved in mouse placenta during pregnancy.

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