Plasmid extraction A+레포트

소개

"Plasmid extraction A+레포트"에 대한 내용입니다.

목차

1. Title
2. Name
3. Purpose
4. Materials
5. Methods
6. Results
7. Discussion
8. References

본문내용

1. Transfer 1㎖ of well-grown bacterial culture to a centrifuge tube.
2. Centrifuge the tube at 13,000rpm for 1 minute to pellet the cells and discard the supernatant.
3. Transfer 1㎖ of well-grown bacterial culture to a centrifuge tube.
4. Centrifuge the tube at 13,000rpm for 1 minute to pellet the cells and discard the supernatant.
5. Transfer remained 1㎖ of well-grown bacterial culture to a centrifuge tube.
6. Centrifuge the tube at 13,000rpm for 1 minute to pellet the cells and discard the supernatant completely.
7. Add 200㎕ of FAPD1 buffer (RNaseA added) to the cell pellet and resuspend the cells completely by pipetting.
8. Add 200㎕ of FAPD2 buffer and gently invert the tube 10 times. Incubate the sample mixture at room temperature for 5 minutes to lyse the cells.
9. Add 300㎕ of FAPD3 buffer and invert the tube 10 times immediately to neutralize the lysate.
10. Centrifuge at 13,000rpm for 8 minutes to clarify the lysate. During centrifugation, place a FAPD column in a collection tube.

참고자료

· 이병무, 『유전자 클로닝과 DNA 분석』, 월드사이언스(2017), 34-38p.
· 이정주, 『유전학원론』, 월드사이언스(2013), 367-374p.
· 이종호, 『의생명과학 실험서 상권』, 라이프사이언스(2021), 26-28, 163-169, 190-202p.