Determination of protein structures

소개

Introduction to Protein Structure (2nd ed.) 책자의
18과를 ppt로 요약하여 발표한 자료입니다.
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목차

Several different techniques
Protein crystals for x-ray crystallography
Crystallization of protein
X-ray sources, either monochromatic or polychromatic
X-ray data are recorded either on image plates or by electronic detectors
Bragg’s law
Three properties of a spot : amplitude, wavelength, phase
Phase determination
Building a model involves subjective interpretation of the data
X-ray diffraction can be used to study the structure of fibers as well as crystals
The structure of biopolymers can be studied using fiber diffraction

본문내용

Several different techniques

Primary : direct determination of the amino acid sequence
from the protein
or indirectly from the necleotide sequence of the
corresponding gene or cDNA
Secondary, Tertiary – X-ray crystallography, NMR
Quaternary – electron microscopy

Protein crystals for x-ray crystallography

Prerequisite : well-ordered structure
regular, repeating array
Diffraction pattern
However, well-ordered crystals are difficult to grow
- irregular surfaces on large, spherical, or ellipsoidal protein
impossible to pack them into a crystal without forming large holes or channels between the individual molecules
a number of different parameters; pH, temp., protein conc., the nature of solvent, precipitant & the presence of added ions or ligands to the protein

참고자료

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