DNA 및 단백질의 전기영동 및 응용

목차

1. Basic principle of electrophoresis
2. Procedure of electrophoresis
3. Types of gel electrophoresis
4. Types of buffer solution for gel electrophoresis
5. Electrophoresis through PAGE
6. Application: DNA profiling

본문내용

Basic principle of electrophoresis
<Electrophoresis>
= movement under electric current.

<Principle>
Nucleic acid or proteins which are charged molecules are separated based on the size difference when subjected to electrical field.

<Factors affecting electrophoresis>
Inherent Factors
• Magnitude of its charge
• Charge density
• Molecular weight
• Tertiary or quaternary structure

External Factors
• pH of buffer
• Strength of electric field
• Viscosity of buffer
• Temperature of tank

참고 자료

없음