가바와 비당체 이소플라본이 증가된 Lactobacillus brevis 발효 콩-분말두유의 생리활성 증진 효과 (Enhanced biological effect of fermented soy-powder milk with Lactobacillus brevis increasing in γ-aminobutyric acid and isoflavone aglycone contents)

서지정보

· 발행기관 : 한국응용생명화학회
· 수록지 정보 : Journal of Applied Biological Chemistry / 61권 / 3호 / 245 ~ 255페이지
· 저자명 : 황정은, 김수철, 이진환, 홍수영, 조계만

초록

본 연구는 Lactobacillus brevis 균주들을 이용하여 제조된 발효콩-분말 두유(fermented soy-power milk, FSPM)의 기능성물질함량(GABA 및 isoflavone)과 항산화 활성 및 소화효소 저해활성을 비교 측정하였다. 김치로부터 가바 생성 유산균 10종을 분리하였고 16S rRNA 유전 분석 결과 L. brevis와 96-99% 상동성을 나타내었다. GABA 전환율은 MSG, 활성 글루텐과 콩 단백질에서 각각 66.96-93.51%, 63.76-84.58%과 57.05-69.75% 있었다. FSPM은 콩-분말 두유(SPM)보다 pH와 GA 함량은 낮았으나, 산도와 GABA 함량은 높았다. BMK484 균주 FSPM의GABA 전환율은 69.97%로 가장 우수하였다. 한편, SPM은 배당체 이소플라본의 함량(1290.93 μg/g)이 높았으나, FSPM는 비당체 이소플라본의 함량(287.27-501.9 μg/g)이 높았다. BMK484 균주 FSPM의 경우 비당체 이소플라본인 daidzein, glycitein과genistein 함량이 각각 240.2, 61.24과 200.45 μg/g으로 가장 높았다. BMK484 균주 FSPM의 DPPH, ABTS와 hydroxyl 라디칼 소거활성 및 α-glucosidase와 pancreatic lipase 저해활성은각 60.31, 88.10와 61.25 및 52.71와 39.37%로 비교적 우수하였다. 따라서 본 연구의 L. brevis들은 발효 콩-분말 두유에서GABA 및 비당체 이소플라본을 동시에 강화할 수 있는 소재로서 사용될 수 있다.

영어초록

The research was aimed to analyze the functional constituents (GABA and isoflavone), radical (2,2-diphenyl-1- picrylhydrazyl (DPPH), 2,2-azino-bis (3-ethylbenzothiazoline-6- sulfonic acid) (ABTS), and hydroxyl) scavenging activities and enzyme (α-glucosidase and lipase) inhibitory effects of soypowder milk (SPM) and fermented soy-powder milk (FSPM) with varied Lactobacillus brevis. Ten γ-aminobutyric acid (GABA) producing lactic acid bacteria that showed 96-99% similarity with L. brevis, according to 16S rRNA gene sequence analysis, were isolated from fermented kimchi. The conversion rates of GABA were obtained 66.96-93.51, 63.76-84.58, and 57.05-69.75% in monosodium glutamate, gluten and soy protein, respectively. The levels of pH and glutamic acid of FSPM were found lower than those of SPM, but the acidity and GABA contents were higher.
The GABA conversion rate of FSPM with BMK484 strain was attained the highest 69.97%. The contents of isoflavone glycoside (1290.93 μg/g) was higher in SPM, but the content of isoflavone aglycone (287.27-501.9 μg/g) was higher in FSPM. The levels of isoflavone aglycone such as daidzein, glycitein and genistein, were found as the highest 240.2, 61.24 and 200.45 μg/g, respectively, when FSPM was made with BMK484 strain. The DPPH, ABTS and hydroxyl radical scavenging and α-glucosidase and pancreatic lipase inhibitory activities of FSPM made with BMK484 strain were the relatively higher 60.31, 88.10, 61.25, 52.71, and 39.37%, respectively. Therefore, the L. brevis can be used as a material capable of simultaneously enhanced GABA and isoflavone aglycone in FSPM.

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