검정콩 함유 안토시아닌의 동시분석을 위한 최적 HPLC 분석 조건 (Optimal HPLC Condition for Simultaneous Determination of Anthocyanins in Black Soybean Seed Coats)

서지정보

· 발행기관 : 한국작물학회
· 수록지 정보 : 한국작물학회지 / 53권 / 4호 / 359 ~ 368페이지
· 저자명 : 정명근

초록

Black soybean has been widely utilized as
foods and oriental medicinal materials. The pigmentation in
the seed coat of black soybean is due to accumulate anthocyanins
in the epidermis palisade layer. The anthocyanins
of black soybean seed coat are considered as a parameter
of quality evaluation of black soybean. Therefore, the purpose
of this study was to investigate the most suitable
HPLC condition for simultaneous determination of anthocyanins
in black soybean seed coats extracts. The efficient
HPLC analytical condition of D3G, C3G, and Pt3G contained
extracts of black soybean seed coats was developed.
The gradient elution employed a 250 mm × 4.6 mm i.d.
YMC-pak ODS-AM 303 column. The gradient system was
used two mobile phases. A gradient elution was performed
with mobile phase A, consisting of 5% aqueous formic
acid, and mobile phase B, comprising 5% formic acid -
acetonitrile, and delivered at a flow rate of 0.7 mL/min as
follows: 0~35 min, 90% A ~ 60% A; 36 min, 90% A; 46
min, 90% A. The UV-VIS. detection wavelength was set
at 520 nm. The limit of detection (LOD) for D3G, C3G,
and Pt3G were under 10 ng/mL.

영어초록

Black soybean has been widely utilized as
foods and oriental medicinal materials. The pigmentation in
the seed coat of black soybean is due to accumulate anthocyanins
in the epidermis palisade layer. The anthocyanins
of black soybean seed coat are considered as a parameter
of quality evaluation of black soybean. Therefore, the purpose
of this study was to investigate the most suitable
HPLC condition for simultaneous determination of anthocyanins
in black soybean seed coats extracts. The efficient
HPLC analytical condition of D3G, C3G, and Pt3G contained
extracts of black soybean seed coats was developed.
The gradient elution employed a 250 mm × 4.6 mm i.d.
YMC-pak ODS-AM 303 column. The gradient system was
used two mobile phases. A gradient elution was performed
with mobile phase A, consisting of 5% aqueous formic
acid, and mobile phase B, comprising 5% formic acid -
acetonitrile, and delivered at a flow rate of 0.7 mL/min as
follows: 0~35 min, 90% A ~ 60% A; 36 min, 90% A; 46
min, 90% A. The UV-VIS. detection wavelength was set
at 520 nm. The limit of detection (LOD) for D3G, C3G,
and Pt3G were under 10 ng/mL.

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