칡소 정액 동결을 위한 AndroMed와 Tris-egg Yolk 희석제의 동결성 비교 (Comparison of AndroMed and Tris-egg Yolk Extender for Cryopreservation of Korean Native Bull Semen (Chick Cow))

서지정보

· 발행기관 : 사단법인 한국동물생명공학회
· 수록지 정보 : 한국동물생명공학회지 / 26권 / 1호 / 65 ~ 70페이지
· 저자명 : 조상래, 김성재, 손준규, 최선호, 최창용, 고응규, 이풍연, 김현종

초록

This study was conducted to investigate the survival rate of AndroMed and Tris-egg yolk extender for cryopreservation of Korean Native Bull Semen (Chick Cow). Semen was collected from a Korean Native Bull Semen over 3 year's old. The semen was diluted 1:1 by AndroMed and Tris-egg yolk extender. The pellet was diluted to final sperm concentration of 5 × 10^7 cell/ml by doubling in every 10 minutes at 4℃ cold chamber. The semen was equilibrated for 1 hrs at cold chamber and packed to 0.5 ml straw. The semen straws were located above 5 cm of liquid nitrogen for 5 minutes. And then the frozen straw was plunged to LN2. The presented straws were examined the viability and motility after thawed at 37℃ water bath. The survival rates was significantly higher (p<0.05) in Tris-egg yolk extender than AndroMed extender (89.7 ± 19.8 vs. 73.4 ± 11.2). However, motility was no significant differences (78.4 ± 18.7 vs. 67.9±14.6). Survival rate in time of equilibration between visual and CASA program had higher in 2 h (86.3 3± 9.4 vs. 92.32 ± 12.4) than in 5 h (78.20 ± 7.8 vs. 88.28 ± 13.1) 15 h (65.24 ± 6.6 vs. 76.48 ± 17.3) 20 h (56.26 ± 4.6 vs. 67.73 ± 18.4). The development rates to cleavage was higher in Tris-egg yolk extender than AndroMed extender (82.2% vs. 81.7%). Similarly, the development rates to blastocyst was significantly higher (p<0.05) in Trisegg yolk extender than AndroMed extender (42.3% vs. 29.6%). In conclusion, the obvious impact of this study will be its practical application to improve viability and fertilizing ability of cryopreserved spermatozoa used for in vitro fertilization (IVF) and AI, Which in turn will be beneficial to animal genetic resources conservation.

영어초록

This study was conducted to investigate the survival rate of AndroMed and Tris-egg yolk extender for cryopreservation of Korean Native Bull Semen (Chick Cow). Semen was collected from a Korean Native Bull Semen over 3 year's old. The semen was diluted 1:1 by AndroMed and Tris-egg yolk extender. The pellet was diluted to final sperm concentration of 5 × 10^7 cell/ml by doubling in every 10 minutes at 4℃ cold chamber. The semen was equilibrated for 1 hrs at cold chamber and packed to 0.5 ml straw. The semen straws were located above 5 cm of liquid nitrogen for 5 minutes. And then the frozen straw was plunged to LN2. The presented straws were examined the viability and motility after thawed at 37℃ water bath. The survival rates was significantly higher (p<0.05) in Tris-egg yolk extender than AndroMed extender (89.7 ± 19.8 vs. 73.4 ± 11.2). However, motility was no significant differences (78.4 ± 18.7 vs. 67.9±14.6). Survival rate in time of equilibration between visual and CASA program had higher in 2 h (86.3 3± 9.4 vs. 92.32 ± 12.4) than in 5 h (78.20 ± 7.8 vs. 88.28 ± 13.1) 15 h (65.24 ± 6.6 vs. 76.48 ± 17.3) 20 h (56.26 ± 4.6 vs. 67.73 ± 18.4). The development rates to cleavage was higher in Tris-egg yolk extender than AndroMed extender (82.2% vs. 81.7%). Similarly, the development rates to blastocyst was significantly higher (p<0.05) in Trisegg yolk extender than AndroMed extender (42.3% vs. 29.6%). In conclusion, the obvious impact of this study will be its practical application to improve viability and fertilizing ability of cryopreserved spermatozoa used for in vitro fertilization (IVF) and AI, Which in turn will be beneficial to animal genetic resources conservation.

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