N,N-Dimethylformamide와 Dimethylsulfoxide를 이용한 감귤 잎의 엽록소 측정방법 확립 (Establishment of Analytical Method for Chlorophyll Using the N,N-Dimethylformamide and Dimethylsulfoxide in Citrus Leaves)

서지정보

· 발행기관 : 한국환경농학회
· 수록지 정보 : 한국환경농학회지 / 33권 / 4호 / 344 ~ 349페이지
· 저자명 : 한승갑, 강석범, 문영일, 박재호, 박경진, 최영훈

초록

BACKGROUND: Purpose of the study was to establish theextinction coefficients of chlorophyll a and b in N,Ndimethylformamide(DMF) and Dimethylsulfoxide(DMSO)solvents and to find out the conditions of optimal extractiontemperature and time in citrus leaves.
METHODS AND RESULTS: Chlorophyll a and bstandards were dissolved in DMF and DMSO. Extinctioncoefficients of chlorophyll pigments were determined andtheir contents were quantified using spectrophotometer.
Chlorophyllous pigments of citrus(Citrus unshiu Marc. cv.
Okitsu wase) leaves were extracted at 25, 40, 60 and 80℃for 4, 6, 8, 24 and 48 hours to determine the optimalextraction condition.
CONCLUSION: The extinction coefficients of chlorophylla(Chl a) and chlorophyll b(Chl b) of DMF extracts for highextinction wavelength were 663.8 and 647.2 nm. Similarly,the high extinction wavelength of DMSO extracts were665.8 and 649.0 nm for chl a and b respectively. Chl a, Chlb and total chlorophyll content of DMF extracts were Chl a= 12.10A663.8 - 2.74A647.2, Chl b = 21.94A647.2 - 5.06A663.8and total Chl = 19.193A647.2 + 7.04A663.8. Similarly, Chl a,Chl b and total Chl of DMSO extracts were Chl a =14.53A665.8 - 5.40A649.0, Chl b = 26.98A649.0 - 7.11A665.8 andtotal Chl = 21.58A649.0 + 7.43A665.8. The chlorophyllextracts of DMF and DMSO were very stable in dark. Highchlorophyll contents of citrus leaves were found at 80℃extraction for 6 hours in DMF and at 80℃ extraction for 24hours in DMSO. However, the chlorophyll content wasdecreased significantly after 8 hours in DMF extractionwhile it was remained up to 30 hours in DMSO extraction.

영어초록

BACKGROUND: Purpose of the study was to establish theextinction coefficients of chlorophyll a and b in N,Ndimethylformamide(DMF) and Dimethylsulfoxide(DMSO)solvents and to find out the conditions of optimal extractiontemperature and time in citrus leaves.
METHODS AND RESULTS: Chlorophyll a and bstandards were dissolved in DMF and DMSO. Extinctioncoefficients of chlorophyll pigments were determined andtheir contents were quantified using spectrophotometer.
Chlorophyllous pigments of citrus(Citrus unshiu Marc. cv.
Okitsu wase) leaves were extracted at 25, 40, 60 and 80℃for 4, 6, 8, 24 and 48 hours to determine the optimalextraction condition.
CONCLUSION: The extinction coefficients of chlorophylla(Chl a) and chlorophyll b(Chl b) of DMF extracts for highextinction wavelength were 663.8 and 647.2 nm. Similarly,the high extinction wavelength of DMSO extracts were665.8 and 649.0 nm for chl a and b respectively. Chl a, Chlb and total chlorophyll content of DMF extracts were Chl a= 12.10A663.8 - 2.74A647.2, Chl b = 21.94A647.2 - 5.06A663.8and total Chl = 19.193A647.2 + 7.04A663.8. Similarly, Chl a,Chl b and total Chl of DMSO extracts were Chl a =14.53A665.8 - 5.40A649.0, Chl b = 26.98A649.0 - 7.11A665.8 andtotal Chl = 21.58A649.0 + 7.43A665.8. The chlorophyllextracts of DMF and DMSO were very stable in dark. Highchlorophyll contents of citrus leaves were found at 80℃extraction for 6 hours in DMF and at 80℃ extraction for 24hours in DMSO. However, the chlorophyll content wasdecreased significantly after 8 hours in DMF extractionwhile it was remained up to 30 hours in DMSO extraction.

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