고속 압출 전처리 공정을 이용한 Chlorella sp. 당화 및 바이오에탄올 생산 (Saccharification and Ethanol Production from Chlorella sp. Through High Speed Extrusion Pretreatment)

서지정보

· 발행기관 : 한국생물공학회
· 수록지 정보 : KSBB Journal / 27권 / 3호 / 137 ~ 144페이지
· 저자명 : 이춘근, 최운용, 서용창, 송치호, 안주희, 정경환, 이상은, 강도형, 이현용

초록

Among various pretreatment processes for bioethanol production, extrusion pretreatment, one of cheap and simple process was investigated to efficiently produce fermentable sugars from micro alga, Chlorella sp. The biomass was pretreated in a single screw extruder at five different barrel temperatures of 45, 50, 55, 60 and 65℃, respectively with five screw rotation speed of 10, 50, 100, 150 and 200 rpm.
The pretreated biomass was reacted with two different hydrolyzing enzymes of cellulase and amyloglucosidase since the biomass contained different types of carbohydrates,compared to cellulose of agricultural by-products such wheat and corn stovers, etc. In general, higher glucose conversion yield was obtained as 13.24 (%, w/w) at 55℃ of barrel temperature and 100 rpm of screw speed conditions.
In treating 5 FPU/glucan of cellulase and 150 Unit/mL of amyloglucosidase, ca. 64% of cellulose and 40% of 1강원대학교 의생명과학대학 의생명소재공학과1Department of Biomedical Materials Engineering, Kangwon National University, Chuncheon 200-701, Korea 2한국교통대학교 보건생명대학 생명공학과2Department of Biotechnology, Korea National University of Transportation, Chungbuk 368-701, Korea 3한국해양과학기술원3Korea Institute of Ocean Science & Technology (KIOST), Ansan P.O. Box 29, Seoul 426-744, Korea 4서원대학교 식품과학부 차학과4Department of Teaics, Seowon University, Cheongju 361-742,Korea Tel: [phone], Fax: [phone]e-mail:[e-mail] polysaccharides in the micro alga were converted into glucose,which was higher yields than those from other reported data without applying an extrusion process. 84% of the fermentable sugars obtained from the hyrolyzing processes were fermented into ethanol in considering 50% of theoretical maximum fermentation yield of the yeast. These results implied that high speed extrusion could be suitable as a pretreatment process for the production of bioethanol from Chlorella sp.

영어초록

Among various pretreatment processes for bioethanol production, extrusion pretreatment, one of cheap and simple process was investigated to efficiently produce fermentable sugars from micro alga, Chlorella sp. The biomass was pretreated in a single screw extruder at five different barrel temperatures of 45, 50, 55, 60 and 65℃, respectively with five screw rotation speed of 10, 50, 100, 150 and 200 rpm.
The pretreated biomass was reacted with two different hydrolyzing enzymes of cellulase and amyloglucosidase since the biomass contained different types of carbohydrates,compared to cellulose of agricultural by-products such wheat and corn stovers, etc. In general, higher glucose conversion yield was obtained as 13.24 (%, w/w) at 55℃ of barrel temperature and 100 rpm of screw speed conditions.
In treating 5 FPU/glucan of cellulase and 150 Unit/mL of amyloglucosidase, ca. 64% of cellulose and 40% of 1강원대학교 의생명과학대학 의생명소재공학과1Department of Biomedical Materials Engineering, Kangwon National University, Chuncheon 200-701, Korea 2한국교통대학교 보건생명대학 생명공학과2Department of Biotechnology, Korea National University of Transportation, Chungbuk 368-701, Korea 3한국해양과학기술원3Korea Institute of Ocean Science & Technology (KIOST), Ansan P.O. Box 29, Seoul 426-744, Korea 4서원대학교 식품과학부 차학과4Department of Teaics, Seowon University, Cheongju 361-742,Korea Tel: [phone], Fax: [phone]e-mail:[e-mail] polysaccharides in the micro alga were converted into glucose,which was higher yields than those from other reported data without applying an extrusion process. 84% of the fermentable sugars obtained from the hyrolyzing processes were fermented into ethanol in considering 50% of theoretical maximum fermentation yield of the yeast. These results implied that high speed extrusion could be suitable as a pretreatment process for the production of bioethanol from Chlorella sp.

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