생녹혈의 건조 및 안정화 (Drying and Stabilization of Deer Blood)

서지정보

· 발행기관 : 한국식품영양학회
· 수록지 정보 : 한국식품영양학회지 / 22권 / 1호 / 20 ~ 28페이지
· 저자명 : 안용근

초록

한방에서는 굳은 녹혈은 약효가 없고, 굳지 않은 생녹혈만
약효가 있다고 하므로 녹혈의 응고를 방지하고, 건조하고 난
후도 물에 다시 녹아서 생리활성을 잃지 않는 녹혈 제조 방법을 개발하였다. 트립신, 펩신, 키모트립신, 아미노펩티다아제
등의 단백질 가수분해효소를 녹혈에 가한 결과, 아무것도 가하지 않은 것과 동일하게 1시간에 녹혈이 응고되었다. 단백질
가수분해효소를 함유한 동아추출액을 가한 것은 31시간 동안
항응고 작용을 나타냈다. 녹혈에 대하여 1% EDTA는 1시간,
0.38% 시트르산 나트륨은 4시간, 0.16% 수산칼슘은 2시간,
1.2% 에탄올은 1시간, 6 ㎎/100 ㎖ 헤파린은 31시간 항응고 작용을 나타냈다. 시트르산나트륨 0.19%+동아추출액 1%와 헤파린 0.006%+동아추출액 1%를 가한 것은 72시간까지 항응고
작용을 나타냈다. 그러나 헤파린은 식품에 사용할 수 없으므로 녹혈에 시트르산 나트륨 0.19%와 동아추출액 1%를 가하여 동결건조하고 타블렛을 제조하였다. 이렇게 만든 타블렛의 물에 녹는 복원률은 96.7%였다. 복원성은 분무건조한 녹혈은 85%, 감압건조한 녹혈은 81%, 시판 녹혈 타블렛은 25.5%였다.
제조한 녹혈 타블렛은 56.4%, 유당 18.7%, 아미노산 1.2%,
글루코오스 1.0%, 지방질 0.7%, 철 180 ㎎/100 g, 칼륨 13 ㎎
/100 g, 칼슘 39.1 ㎎/100 g, 나트륨 480 ㎎/100 g, 염소 368 ㎎
/100 g을 나타냈다.

영어초록

According to traditional oriental medicine, only non-coagulated native deer blood is said to be effective, and coagulated deer blood is ineffective. Thus, a drying and tablet-producing method for deer blood was developed to maintain its physiological and therapeutic activity, and so that after drying, it can be redissolved and protected from coagulation. Proteases such as trypsin, pepsin, chymotrypsin, and aminopeptidase were added to the deer blood indicating that it coagulated in an hour, as shown by the reference. Wax gourd extract, which is high in protease, was added to the blood resulting in anticoagulation for 31 hours. Also, additions of 1% EDTA, 0.38% sodium citrate, 0.16% calcium oxalate, 1.2% ethanol, and 0.006% heparin to the deer blood resulted in anticoagulation for 1 hour, 4 hours, 2 hours, 1 hour, and 31 hours, respectively. In an experiment using 0.19% sodium citrate plus 1% wax gourd extract, and 0.006% heparin plus 1% wax gourd extract, anticoagulation was maintained for up to 72 hours. However, since heparin can not be used in food, the deer blood tablet was made with the addition of 0.19% sodium citrate and 1% wax gourd extract, followed by freeze drying. The dissolution rate for the tablet manufactured in this manner was 96.7%. And the dissolution rates for spray-dried deer blood, vacuum-dried deer blood, and marketed deer blood tablets were 85%, 81%, and 25.5%, respectively. The composition of the tablet produced from the freeze-dried deer blood was 56.4% protein, 18.7% lactose, 1.2% amino acids, 1.0% glucose, 0.7% lipids, 180 ㎎/100 g of iron, 13 ㎎/100 g of potassium, 39.1 ㎎/100 g of calcium, 480 ㎎/100 g of sodium, 368 ㎎/100 g of chloride, each.

참고자료

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