호주산 Propolis의 항균활성과 치주조직에 미치는 영향 (Antimicrobial Activity and the Effect on Periodontium of Australian Propolis)

서지정보

· 발행기관 : 한국생체재료학회
· 수록지 정보 : 생체재료학회지 / 12권 / 3호 / 102 ~ 108페이지
· 저자명 : 주동옥, 국중기, 김병옥, 김생곤

초록

Propolis is a naturally-occurring bee products and has been used in folk medicine for several purposes such as an antiseptic,
anti-inflammatory, antimycotic and bacteriostatic agent. The aim of the present work was to evaluate the antibacterial
activity and periodontal effects of Australian propolis (Green-One natural health, Sydney, Australia).
Antibacterial effect of diluted propolis on seven oral pathogens, Prevotella intermedia (Pi), Prevotella nigrescens (Pn),
Porphyromonans endodontalis (Pe), Porphyromonas gingivalis (Pg), Tannerella forsythensis (Tf) Actinobillus actinomycetemcomitans
(Aa), and Fusobacterium nucleatum (Fn), were tested and their cytotoxic effect on human gingival
fibroblasts (hGF) and periodontal ligament fibroblasts (hPDLF) were evaluated by MTT. Cell viability assays were performed
using third to fourth-passage of hGF and hPDGF. And, for investigating the periodontal effect of propolis, gingival
creviculr fluid (GCF) and sulcular bleeding index (SBI) were measured 10-day after applying diluted propolis with
periodontal pack. MTT assay had demonstrated that 1/27 dilution of propolis had antimicrobial effect and was safe for
hGF and hPDLF. After applying diluted propolis with periodontal pack, GCF was significantly reduced but SBI showed
decreasing tendency. Author confirms that propolis has antiseptic, antibacterial and antiinflammatory properties if
appropriate dilution can be prepared. And, more researches are needed to find out the appropriate diluted propolis
solutions which are less cytotoxic for tissue cells but present strong antibacterial properties, and promote the wound
healing.

영어초록

Propolis is a naturally-occurring bee products and has been used in folk medicine for several purposes such as an antiseptic,
anti-inflammatory, antimycotic and bacteriostatic agent. The aim of the present work was to evaluate the antibacterial
activity and periodontal effects of Australian propolis (Green-One natural health, Sydney, Australia).
Antibacterial effect of diluted propolis on seven oral pathogens, Prevotella intermedia (Pi), Prevotella nigrescens (Pn),
Porphyromonans endodontalis (Pe), Porphyromonas gingivalis (Pg), Tannerella forsythensis (Tf) Actinobillus actinomycetemcomitans
(Aa), and Fusobacterium nucleatum (Fn), were tested and their cytotoxic effect on human gingival
fibroblasts (hGF) and periodontal ligament fibroblasts (hPDLF) were evaluated by MTT. Cell viability assays were performed
using third to fourth-passage of hGF and hPDGF. And, for investigating the periodontal effect of propolis, gingival
creviculr fluid (GCF) and sulcular bleeding index (SBI) were measured 10-day after applying diluted propolis with
periodontal pack. MTT assay had demonstrated that 1/27 dilution of propolis had antimicrobial effect and was safe for
hGF and hPDLF. After applying diluted propolis with periodontal pack, GCF was significantly reduced but SBI showed
decreasing tendency. Author confirms that propolis has antiseptic, antibacterial and antiinflammatory properties if
appropriate dilution can be prepared. And, more researches are needed to find out the appropriate diluted propolis
solutions which are less cytotoxic for tissue cells but present strong antibacterial properties, and promote the wound
healing.

참고자료

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