보리의 발아를 통한 Phytase 생산과 특징 (The Production and Characterization of Phytase by Germination in Barley)

서지정보

· 발행기관 : 강원대학교 농업생명과학연구원
· 수록지 정보 : 농업생명환경연구 / 33권 / 3호 / 329 ~ 338페이지
· 저자명 : 성하균

초록

As a study for producing abundantly activated phytase in the germinated barleys, which are grain feed materials, we investigated the changes and characteristics of phytase during germination in barley. When enzyme activity of phytase was measured after germination of corn, wheat, barley and rice under the same conditions as grains that can be used as feed materials, germinated barley showed the highest phytase activity of 2.91 ± 0.07 U/g compared to other grains (p < 0.05). The phytase activity of barley gradually increased with the times of germination, and decreased after peaking at 3.00 ± 0.32 U/g on the fourth day of germination. The temperature and pH of germinated barley phytase showed the highest peaks at 55°C and pH 5.0. Addition of 0.01% Tween 80 showed the best effects on the phytase activity of germinated barley. In all experiments, phytase activity had a tendency to decrease compared to the initial activity as the reaction time elapsed, but the treatment group of 0.01% Tween 80 maintained a similar level until 30 minutes after the reaction. Therefore, these results are considered to provide basic data for using germinated barley to improve the digestibility of feed-derived phytase and to prevent phosphate contamination of the environment through fecal excretion.

영어초록

As a study for producing abundantly activated phytase in the germinated barleys, which are grain feed materials, we investigated the changes and characteristics of phytase during germination in barley. When enzyme activity of phytase was measured after germination of corn, wheat, barley and rice under the same conditions as grains that can be used as feed materials, germinated barley showed the highest phytase activity of 2.91 ± 0.07 U/g compared to other grains (p < 0.05). The phytase activity of barley gradually increased with the times of germination, and decreased after peaking at 3.00 ± 0.32 U/g on the fourth day of germination. The temperature and pH of germinated barley phytase showed the highest peaks at 55°C and pH 5.0. Addition of 0.01% Tween 80 showed the best effects on the phytase activity of germinated barley. In all experiments, phytase activity had a tendency to decrease compared to the initial activity as the reaction time elapsed, but the treatment group of 0.01% Tween 80 maintained a similar level until 30 minutes after the reaction. Therefore, these results are considered to provide basic data for using germinated barley to improve the digestibility of feed-derived phytase and to prevent phosphate contamination of the environment through fecal excretion.

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