발효 청미래덩굴잎 용매 추출물 및 분획물의 xanthine 및 aldehyde oxidase 저해활성과 항고요산혈증 효과 (Xanthine and Aldehyde Oxidase Inhibitory Activities, and Antihyperuricemic Effects of Fermented Smilax china L. Leaf Extracts and Fractions)

서지정보

· 발행기관 : 한국응용생명화학회
· 수록지 정보 : Journal of Applied Biological Chemistry / 57권 / 1호 / 53 ~ 59페이지
· 저자명 : 이상일, 이예경, CHENGJINHUA, 양승환, 서주원, 김순동

초록

Aspergillus oryzae로 발효시킨 청미래덩굴잎 용매추출분획물의xanthine oxidase (XO) 및 aldehyde oxidase (AO) 저해활성과항고요산혈증에 미치는 영향을 조사하였다. 용매별 추출수율(g/kg)은 80% ethanol (EtOH)은 13.56이었으며, n-hexane, dichloromethane(DICM), ethylacetate (EtOAc) 및 n-butanol fraction (BuOH)은 1.35−3.33 범위였다. Total polyphenol 함량(mg/g-extract)은EtOAc fraction 478.07−501.26, BuOH fraction 259.49−289.02,DICM fraction 165.03−232.27, EtOH fraction 134.02−196.54이었으며 EtOAc 및 DICM fraction에서는 발효시킨 경우가 비발효에 비하여 각각 4.85 및 40.74%가 높았으나 그 외 모든fraction에서는 발효시킨 경우가 낮았다.
총 flavonoid 함량은 EtOAc fraction이 여타 fraction에 비하여 높았다. EtOAc 및 BuOH fraction의 TF 함량은 발효시킨경우가 비 발효에 비하여 각각 10.56% 및 60.17%가 높았으나여타 fraction들에서는 발효시킨 경우가 낮았다. XO 저해활성은모든 분획물에서 발효시킨 경우가 비발효에 비하여 현저하게 높았으며 EtOAc 및 BuOH fraction의 경우 발효시킨 경우는 각각 75.02 및 65.59%로 비발효 경우 39.42 및 5.34% 보다 현저하게 높았다. AO 저해활성은 DICM과 EtOAc fraction에서각각 81.82 및 77.93%로 여타 fraction들에 비하여 높았으며XO의 경우와 마찬가지로 발효시킨 경우가 비발효에 비하여 현저하게 높았다. 고요산혈증 mouse의 혈중 요산(SU) 함량에 미치는 영향을 조사한 결과, 고요산혈증 대조군의 SU 함량은 6.98mg/dL로 정상대조군 (NC)의 3.82 mg/dL에 비하여 1.83배가 높았으나, EtOAc fraction은 투여량에 의존적으로 SU의 함량이감소하였으며, 발효시킨 경우가 비발효에 비하여 유의적인 감소를 보였다. 이상의 결과, A. oryzae와 청미래덩굴잎의 발효과정을 통해, glycoside형태의 flavonoid를 aglycone화하여 비발효에비해 높은 XO 및 AO 저해활성과 함께 항고요산혈증 효과를나타내는 것으로 사료된다.

영어초록

To evaluate the inhibitory effect of xanthine oxidase(XO) and aldehyde oxidase (AO), and antihyperuricemic effect byAspergillus oryzae fermented Smilax china L. leaf extracts andfractions, we observed extracted yield by each solvent, the contentof total polyphenol and total flavonoid (TF), the activities of XOand AO, and serum uric acid level. Extracted yield (g/kg) by 80%ethanol (EtOH) was 13.56, those of n-hexane, dichloromethane(DICM), ethylacetate (EtOAc) and n-butanol fraction (BuOH)were 1.35−3.33. Furthermore, total polyphenol content (mg/gextract)of EtOAc fraction, BuOH fraction, DICM fraction andEtOH fraction is 478.07−501.26, 259.49−289.02, 165.03−232.27,134.02−196.54, respectively. Those of fermented EtOAc andDICM fraction was 4.85 and 40.74% higher than that of nonfermentedfraction, respectively, while the other fermented fractionswere lower than those of non-fermented fractions. And totalflavonoid content (mg/g-extract) of EtOAc fraction was higherthan those of other fractions. Additionally, TF of fermentedEtOAc and BuOH fraction is 10.56 and 60.17% higher, than thatof fermented fraction, respectively, although those of the otherfermented fractions was lower than that of non-fermented fractions.
On the other hand, XO inhibitory activities of all fermentedfractions was significantly higher than that of all non-fermentedfraction, while those of fermented EtOAc (75.02%) and BuOHfraction (65.59%) was markedly higher than that of non-fermentedfraction (39.42 and 5.34%), respectively. In addition, AO inhibitoryactivities of DICM and EtOAc fraction was 81.82 and 77.93%higher, respectively, than those of the other fractions, and those offermented fractions as with XO were significantly higher than thatof non-fermented fractions. Meanwhile, serum uric acid level(SU) of hyperuricemic control mice (HC, 6.98 mg/dL) was 1.83folds higher than that of normal control (NC, 3.82 mg/dL).
Furthermore, SU in the group treated with EtOAc fraction decreasedin a dose dependent manner compared with the allopurinol controlgroup, although those of fermented fractions were significantlylower than those of non-fermented fractions. This study suggeststhat fermented Smilax china L. leaf extracts may regulate the XOand AO inhibitory activities and antihyperuricemic effect due toaglycone components from glycoside form flavonoids byfermentation of A. oryzae.

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