좁은잎산사나무 열매 추출물의 효능에 관한 연구 (A Study of Effects of Crataegus pinnatifida var. psilosa Extracts)

서지정보

· 발행기관 : 한국식품영양학회
· 수록지 정보 : 한국식품영양학회지 / 31권 / 6호 / 775 ~ 782페이지
· 저자명 : 이광수, 박경숙

초록

The purpose of this study was to investigate effects of Crataegus pinnatifida var. psilosa extracts obtained from 95% methanol and water. Methylene chloride, ethyl acetate, and methanol were used to fractionate the 95% methanol extract and each fraction was testified total polyphenol contents, electron donating abilities, the scavenging abilities of superoxide anion radical, as well as hydrogen peroxide. Extraction yields of 95% methanol and water from Crataegus pinnatifida var.
psilosa were 25.40%, 23.12% respectively. Total polyphenol contents were 28,708.0±1,755.05 μg GAE/mL in 95% methanol, revealing the highest among them, 12,726.67±479.33 μg GAE/mL in water extract, 15,854.67±498.38 μg GAE/mL in methanol fraction, 11,810.67±584.48 μg GAE/mL in ethyl acetate fraction, and 5294.67±190.36 μg GAE/mL in methylene chloride fraction. Total polyphenol contents revealed significant differences (p<0.05) between the solvents. In the experiment of the electron donating ability, water extract revealed 84.33±0.1% scavenging ability, the highest. Other extracts and fractions were 81.8±1.11% for water, 79.73±1.32% for ethyl acetate fraction, 75.73±2.17% for methylene chloride fraction, and 42.1±5.01% for methanol fraction, the lowest electron donating ability. Electron donating abilities revealed significant difference (p<0.05) between the solvents. In the experiment of superoxide anion radical scavenging ability, ethyl acetate fraction(0.0026±0.0002) had the highest scavenging ability, and the others revealed slight increase rather than decrease in scavenging ability. Hydrogen peroxide scavenging ability revealed the highest in methanol fraction (－0.00206±0.00165) and the others were as follow; water extract (0.00157±0.00249), 95.0% methanol extract (0.005±0.0036), methylene chloride fraction (0.0039±0.00364), and ethyl acetate fraction (0.0002±0.00059).

영어초록

The purpose of this study was to investigate effects of Crataegus pinnatifida var. psilosa extracts obtained from 95% methanol and water. Methylene chloride, ethyl acetate, and methanol were used to fractionate the 95% methanol extract and each fraction was testified total polyphenol contents, electron donating abilities, the scavenging abilities of superoxide anion radical, as well as hydrogen peroxide. Extraction yields of 95% methanol and water from Crataegus pinnatifida var.
psilosa were 25.40%, 23.12% respectively. Total polyphenol contents were 28,708.0±1,755.05 μg GAE/mL in 95% methanol, revealing the highest among them, 12,726.67±479.33 μg GAE/mL in water extract, 15,854.67±498.38 μg GAE/mL in methanol fraction, 11,810.67±584.48 μg GAE/mL in ethyl acetate fraction, and 5294.67±190.36 μg GAE/mL in methylene chloride fraction. Total polyphenol contents revealed significant differences (p<0.05) between the solvents. In the experiment of the electron donating ability, water extract revealed 84.33±0.1% scavenging ability, the highest. Other extracts and fractions were 81.8±1.11% for water, 79.73±1.32% for ethyl acetate fraction, 75.73±2.17% for methylene chloride fraction, and 42.1±5.01% for methanol fraction, the lowest electron donating ability. Electron donating abilities revealed significant difference (p<0.05) between the solvents. In the experiment of superoxide anion radical scavenging ability, ethyl acetate fraction(0.0026±0.0002) had the highest scavenging ability, and the others revealed slight increase rather than decrease in scavenging ability. Hydrogen peroxide scavenging ability revealed the highest in methanol fraction (－0.00206±0.00165) and the others were as follow; water extract (0.00157±0.00249), 95.0% methanol extract (0.005±0.0036), methylene chloride fraction (0.0039±0.00364), and ethyl acetate fraction (0.0002±0.00059).

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