꾸지뽕나무 열매에서 추출한 수용성 다당류의 구조분석 (Structure Analysis of Water-soluble Polysaccharides Extracted from The Unripe Fruit of Cudrania tricuspidata)

서지정보

· 발행기관 : 한국목재공학회
· 수록지 정보 : 목재공학 / 42권 / 6호 / 740 ~ 746페이지
· 저자명 : 김석주, 이경태, 유원재, 이성숙, 김용식

초록

꾸지뽕나무 미숙과를 50% 발효주정으로 추출하고, 농축한 추출용액을 n-hexane, chloroform, ethyl acetate, butanol로순차적으로 분액하여 수용성 조추출물을 얻었다. 수용성 조추출물을 분취용 겔 투과 크로마토그래피(Gel PermeationChromatography, GPC)를 실시하여 다당류(F1)를 분리하였다. F1을 가수분해하여 단당 성분 분석을 실시한 결과 glucose,galactose, arabinose, xylose 등 4가지로 구성되어 있었으며, 그 조성비는 6.1 : 1.8 : 3.1 : 1.0이었다. F1에 acetyl기의 치환여부와 uronic acid의 존재 유무를 확인하기 위해 FT-IR로 측정하고 염산 가수분해 한 다음 HPLC 분석을실시한 결과, 두 가지 모두 없는 것으로 분석되었다. 결합 위치 확인을 위해 permethylation, 산 가수분해, 환원, silylation을순차적으로 실시하여 얻은 partially methylated alditol silylate (PMAS)들을 GC-MS 분석을 실시한 결과,1,5-di-O-trimethylsilyl-2,3,4-tri-O-methyl xylose, 1,5-di-O-trimethylsily-2,3,4-tri-O-methyl arabinose, 1,4,5-tri-O-trimethylsily-2,3-di-O-methy arabinose, 1,3,5-tri-O-trimethylsily-2,4,6-tri-O-methyl glucose, 1,4,5-tri-O-trimethylsily-2,3,6-tri-O-methyl galactose, 1,4,5-tri-O-trimethylsily-2,3,6-tri-O-methyl glucose, 1,3,5,6-tetra-O-trimethylsily-2,4-di-O-methyl galactose,1,3,5,6-tetra-O-trimethylsily-2,4-di-O-methyl glucose로 구성되어 있었고 그 조성비는 1.1 : 1.0 : 4.9 : 7.5 : 3.0: 3.1 : 1.4 : 1.5였다.

영어초록

The unripe fruit of cudrania tricuspidata was extracted with 50% ethanol. The crude water-soluble extracts wereseparated by liquid-liquid separation with n-hexane, ethyl acetate and butanol followed by precipitation with ethanol,and then the water-soluble polysaccharide (F1) was isolated by the fractionation through gel permeation chromatographyusing preparative PLaquagel-OH column with water. The structure was characterized by monosaccharide compositionwith HPAEC-PAD, methylation analysis with GC-MS, FT-IR and HPLC. According to the data, F1 was composed of glucose (22.84 mM), galactose (13.75 mM), arabinose (45.87 mM), xylose (7.49 mM). It was revealed which uronic acid and acetyl group were not attached in F1. And it is constituted of 1-linked arabinose, 1,4-linked arabinose,1,3-linked glucose, 1,4-linked galactose, 1,4-linked glucose, 1,3,6-linked galactose, 1,3,6-linked glucose and the ratiowas showed 1.1 : 1.0 : 4.9 : 7.5 : 3.0 : 3.1 : 1.4 : 1.5.

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