GC-MS를 이용한 건강기능식품 중 옥타코사놀 분석법 개발 연구 (Optimization of analytical methods for octacosanol in related health functional food with GC-MS)

서지정보

· 발행기관 : 한국식품위생안전성학회
· 수록지 정보 : 한국식품위생안전성학회지 / 33권 / 4호 / 266 ~ 271페이지
· 저자명 : 이진희, 오미현, 이경진, 김양선, 금은희, 박지은, 조미현, 성민혜, 김상아, 김미혜

초록

The Ministry of Food and Drug Safety (MFDS) is amending its test methods for health-functional foods (dietary food supplements) to establish regulatory standards and specifications in Korea. In this regard, we continue our research on developing analytical methods for the items.
Octacosanol is the major component of polycosanol andis a high-molecular-mass primary fatty alcohol, obtained from sugar cane wax. Previous researchers have shown that octacosanol can lower cholesterol and has antiaggregatory properties, cytoprotective uses, and ergogenic properties for human health. Recently, octacosanol products have been actively introduced into the domestic market because of their functional biological activity.
We have developed a sensitive and selective test method for octacosanol that the TMS derivatives by means of gas-chromatographictandem mass spectrometry (GC-MS).The trimethylsilyl ether derivative of the target analyte showed excellent chromatographic properties. The procedure was validated in the range of 12.5 ~ 200 ㎍/ℓ. Standard calibration curves presented linearity with the correlation coefficient (r2) > 0.999, and the limits of detection (LOD) and limits of quantitation (LOQ) were 4.5 ㎍/L and 13.8 ㎍/L, respectively. The high recoveries (92.5 to 108.8 %) and precision (1.8 to 2.4 %) obtained are in accordance with the established validation criteria. Our research can provide scientific evidence to amend the octacosanol test method for the Health-Functional Food Code.


We have developed a sensitive and selective test method for octacosanol that the TMS derivatives by means of gas-chromatographic-tandem mass spectrometry (GC-MS). The trimethylsilyl ether derivative of the target analyte showed excellent chromatographic properties. The procedure was validated in the range of 12.5 ~ 200 ㎍/ℓ. Standard calibration curves presented linearity with the correlation coefficient (r2) > 0.999, and the limits of detection (LOD) and limits of quantitation (LOQ) were 4.5 ㎍/L and 13.8 ㎍/L, respectively. The high recoveries (92.5 to 108.8 %) and precision (1.8 to 2.4 %) obtained are in accordance with the established validation criteria. Our research can provide scientific evidence to amend the octacosanol test method for the Health-Functional Food Code.

영어초록

The Ministry of Food and Drug Safety (MFDS) is amending its test methods for health-functional foods (dietary food supplements) to establish regulatory standards and specifications in Korea. In this regard, we continue our research on developing analytical methods for the items.
Octacosanol is the major component of polycosanol andis a high-molecular-mass primary fatty alcohol, obtained from sugar cane wax. Previous researchers have shown that octacosanol can lower cholesterol and has antiaggregatory properties, cytoprotective uses, and ergogenic properties for human health. Recently, octacosanol products have been actively introduced into the domestic market because of their functional biological activity.
We have developed a sensitive and selective test method for octacosanol that the TMS derivatives by means of gas-chromatographictandem mass spectrometry (GC-MS).The trimethylsilyl ether derivative of the target analyte showed excellent chromatographic properties. The procedure was validated in the range of 12.5 ~ 200 ㎍/ℓ. Standard calibration curves presented linearity with the correlation coefficient (r2) > 0.999, and the limits of detection (LOD) and limits of quantitation (LOQ) were 4.5 ㎍/L and 13.8 ㎍/L, respectively. The high recoveries (92.5 to 108.8 %) and precision (1.8 to 2.4 %) obtained are in accordance with the established validation criteria. Our research can provide scientific evidence to amend the octacosanol test method for the Health-Functional Food Code.


We have developed a sensitive and selective test method for octacosanol that the TMS derivatives by means of gas-chromatographic-tandem mass spectrometry (GC-MS). The trimethylsilyl ether derivative of the target analyte showed excellent chromatographic properties. The procedure was validated in the range of 12.5 ~ 200 ㎍/ℓ. Standard calibration curves presented linearity with the correlation coefficient (r2) > 0.999, and the limits of detection (LOD) and limits of quantitation (LOQ) were 4.5 ㎍/L and 13.8 ㎍/L, respectively. The high recoveries (92.5 to 108.8 %) and precision (1.8 to 2.4 %) obtained are in accordance with the established validation criteria. Our research can provide scientific evidence to amend the octacosanol test method for the Health-Functional Food Code.

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