Catalog of Lactic Acid Bacteria Associated with Vegetable Sprouts

서지정보

· 발행기관 : 경상대학교 농업생명과학연구원
· 수록지 정보 : 농업생명과학연구 / 57권 / 6호
· 저자명 : Ok Hee Choi, Won Il Kim, Dae Young Son, Ye Yeong Lee, Yong Sung Kang, Jin Woo Kim

목차

Introduction
Materials and Methods
1. Seed sprouting
2. LAB isolation
3. Assay of LAB antibacterial activity against S. enterica
4. 16S rRNA gene sequencing
5. LAB suppression of S. enterica growth in co-culture
6. LAB suppression of S. enterica growth in alfalfasprouts
Results and Discussion
1. Sprout-borne LAB
2. LAB suppressed S. enterica growth in co-culture
3. LAB did not suppress S. enterica growth inalfalfa sprouts
Acknowlegement
References

영어초록

In this study, we examined the antagonistic effects of sprout-borne lactic acid bacteria (LAB) on Salmonella enterica serovar Enteritidis. This antagonism is promoted as a means of controlling contamination during sprout production and provides additional LAB for consumers. We isolated a total of 24 LAB isolates in nine species and five genera from seven popular vegetable sprouts: alfalfa (Medicago sativa), clover (Trifolium pratense), broccoli (Brassica oleracea ssp. italica), vitamin (B. rapa ssp. narinosa), red radish (Raphanus sativus), red kohlrabi (B. oleracea var. gongylodes), and Kimchi cabbage (B. campestris var. pekinensis). Based on 16S rRNA gene sequences, the LAB species were identified as Enterococcus casseliflavus, E. faecium, E. gallinarum, E. mundtii, Lactococcus taiwanensis, Leuconostoc mesenteroides, Pediococcus pentosaceus, and Weissella cibaria, and W. confusa. A total of 16 LAB isolates in seven species including E. faecium, E. gallinarum, E. mundtii, L. taiwanensis, L. mesenteroides, P. pentosaceus, and W. cibaria showed antagonistic activity toward S. enterica. The growth inhibition of sprout LAB on S. enterica was confirmed by co-culture. Unexpectedly, sprout LAB failed to suppress the growth of S. enterica in alfalfa sprouts, whereas all LAB strains stimulate S. enterica growth even if it is not significant in some strains. The findings of this study indicate that S. enterica-antagonistic LAB are detrimental to food hygiene and will contribute to further LAB research and improved vegetable sprout production.

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