Unveiling the potent antioxidant potential of Tulsi (Ocimum sanctum) leaf and stem extract through multi-method screening

서지정보

· 발행기관 : 한국예방수의학회(구 한국수의공중보건학회)
· 수록지 정보 : 예방수의학회지 / 47권 / 3호
· 저자명 : Most Nusrat Zahan, Jong Hyuk Kim, Hyeryeon Yang, Sin Yeong Park, Min Yeong Kim, Il Rae Rho, Euikyung Kim, Changkeun Kang

목차

INTRODUCTION
MATERIALS AND METHODS
Preparation of Tulsi leaf extract (TLE) and Tulsi stemextract (TSE)
Measurement of total polyphenol content
DPPH radical scavenging assay
Reducing power assay
Quantification of eugenol content in TLE using HPLCanalysis
Statistical analysis
RESULTS
Determination of total Polyphenol contents of TLEand TSE
Determination of radical scavenging activities of TLEand TSE
Determination of total ferric reducing ability of TLEand TSE
Determination of eugenol content in TLE using HPLCanalysis
DISCUSSION

영어초록

Tulsi (Ocimum sanctum), commonly known as Holy Basil is a revered herb with a rich history in traditional medicine systems, particularly in Southeast Asia. For its medicinal properties, Tulsi has been regarded as an “Elixir of Life” and has been used to treat various ailments. However, the comprehensive investigation of Tulsi extracts and their potential pharmacological benefits, specifically in relation to antioxidant activity remains limited. Hence, the objective of this study was to evaluate the antioxidant activity of Tulsi leaf and stem extract using various screening methods. We investigate the antioxidant activity exhibited by the extract using three different methods involved the utilization of the total polyphenol content assay, the ferric reducing power assay and 2, 2-diphenyl-1-14 picrylhydrazyl (DPPH) assays. The results revealed that the Tulsi leaf extract (TLE) exhibited significantly higher antioxidant activity when compared to the Tulsi stem extract (TSE) in all the performed assays. The higher content of phenolics in TLE may have contributed to its superior antioxidant activity. The HPLC (high performance liquid chromatography) analysis of TLE revealed the presence of eugenol, active compound for several therapeutic properties. These findings provide an understanding of the bioactive compounds present in Tulsi extracts and their potential antioxidant benefits.

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