Vaccination of Brucella abortus recombinant protein DapB induces CD4+/CD8+ T cells differentiation during Brucella abortus infection in BALB/c mice

서지정보

ㆍ발행기관 : 한국예방수의학회(구 한국수의공중보건학회) ㆍ수록지정보 : 예방수의학회지 / 47권 / 3호
ㆍ저자명 : Tran Xuan Ngoc Huy, Ched Nicole Turbela Aguilar, Trang Thi Nguyen, Said Abdi Salad, Hu Jang Lee, Jin Hur, Dong-Kwan Lee, Suk Kim

목차

INTRODUCTION
MATERIALS AND METHODS
Bacterial strains and growth condition
Recombinant protein expression and purification
Western blotting for immunoreactivity of recombinantproteins
Mice immunization and bacterial challenge
Samples collection and protection level analysis in vivo
Quantitative measurement of CD4+ and CD8+ T cells
Statistical analysis
RESULTS
Protein purification and immunoreactivity of recombinantproteins
Peripheral blood CD4+ and CD8+ T cell subsetsproliferation
Protective effects of vaccination against B. abortus inmice
DISCUSSION
ACKNOWLEDGEMENTS

영어 초록

Extensive research and testing continue to be conducted for the development of vaccines targeting zoonotic diseases such as brucellosis. In this study, the potential of the DapB as a recombinant protein vaccine to effectively combat Brucella abortus 544 infection in BALB/c mice was evaluated. Western blotting assay results showed that recombinant protein DapB reacted with Brucella-positive serum, indicating its potential immunoreactivity. In vivo results showed that the peripheral blood CD4+ and CD8+ T cell population significantly increased in the DapB-immunized mice group after the first, second and third blood collection, compared to the control group that received PBS. Additionally, at the fourth blood collection, an increase in CD4+ T cell activation was observed in three vaccination groups compared to PBS negative control group. These results indicate the potential of DapB in stimulating cellular immunity. Fourteen days after infection, the bacterial load in the spleen was evaluated. The reduction in bacterial replication in the spleen by both DapB and RB51 highlights their protective efficacy against Brucella infection. These findings contribute to the ongoing efforts in developing effective vaccines against brucellosis and provide valuable insights for further research in this field.

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