Development of a Multiplex Polymerase Chain Reaction Method for Simultaneous Detection of Genetically Modified Soy and Maize

서지정보

· 발행기관 : 한국식품위생안전성학회
· 수록지 정보 : 한국식품위생안전성학회지 / 25권 / 3호
· 저자명 : Kyoung Sik Park, Mi-Gyeong Kim, Donggil Leem, Taehyung Yoon, Ki-Mi No, Jin Hong, Eun Mi Kwon, Ae Rie Moon, Jayoung Jeong

목차

ABSTRACT
Materials and Methods
Materials
Preparation of test samples and DNA extraction
Oligonucleotide primers
Analysis of PCR products
Results and Discussion
Acknowledgment
References

영어초록

This study was aimed to develop a novel qualitative multiplex polymerase chain reaction (PCR) for simultaneous detection of genetically modified (GM) soy and maize within a single reaction. The specific primers designed to detect four respective GM events (A2704-12, MON88017, Bt11, and MON863) were included in the tetraplex PCR system. Each of PCR products for four GM events could be distinguished by agarose gel based on their different lengths. The specificity and reproducibility of this multiplex PCR were evaluated. This multiplex PCR consistently amplified only a fragment corresponding to a specific inserted gene in each of the four GM events and also amplified all four of the PCR products in the simulated GM mixture. These results indicate that this multiplex PCR method could be an effective qualitative detection method for screening GM soy and maize in a single reaction.

참고자료

· 없음