DNA Degradation이 Genotyping-by-Sequencing(GBS) 자료 기반 한우 유전적 표지 발굴에 미치는 영향

서지정보

· 발행기관 : 강원대학교 동물자원공동연구소
· 수록지 정보 : 동물자원연구 / 28권 / 4호
· 저자명 : 유동안, 김권도, 김덕경, 조서애

목차

ABSTRACT
Ⅰ. 서론
Ⅱ. 재료 및 방법
1. Genotype-by-sequencing 을 통한 단편서열 생산
2. 단편염기서열 자료 정렬을 통한 SNP발굴
Ⅲ. 결과 및 고찰
1. Agarose gel 전기영동을 통한 DNA 품질 비교
2. Genotype-by-sequencing을 통한 단편서열 생산
3. 참조 유전체 정렬을 통한 SNP발굴
Ⅳ. 요약
사사
Ⅴ. 참고문헌

영어초록

Genotyping-by-sequencing (GBS) is a cost-efficient method which can be useful for SNP marker discovery in a population of interest. GBS is genome reduction sequencing method using restriction enzyme. The quality of DNA is a key factor which could have an influence in downstream analysis. However, there have not been many studies which investigated the impact of DNA degradation and the quality of the data on marker discovery. In this study, GBS data of 6 Hanwoo samples (H1~6) showing differing level of DNA degradation were compared. Re-sequencing pipeline was followed to investigate the impact of DNA degradation on marker discovery. As a result, we found that the quantity and quality of SNPs were not affected in the sample H5 and H6 with moderately degraded DNA. On the other hand, marker discovery was greatly affected in samples with severe DNA degradation (H3 and H4). The findings in this study support that GBS is a robust genotyping method towards moderate DNA degradation.

참고자료

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