DateAseptic Technique and Transfer of MicroorganismGroup4분반학번Table of ContentsAbstract and Introduction4Apparatus4Reagents5Procedures6Procedures6Aseptic technique and transferring microorganism (pg.6)Optimal growth temperature assay (pg.6)Contamination assay (pg.6)data7Aseptic technique and transferring microorganism (7~10)Mueller Hinton Broth tube (pg.7)Mueller Hinton Agar slant tube (pg.8)Mueller Hinton Agar stab tube (pg.9)Mueller Hinton Agar plate (pg.10)Optimal growth temperature assay11Contamination assay12result13Aseptic technique and transferring microorganism (13~14)Result (pg.13)Mueller Hinton slant tubeMueller Hinton Agar plateObserved (pg.14)Mueller Hinton slant tubeMueller Hinton Agar plateOptimal growth temperature assay(15~16)Contamination assay(17~18)Review questions19Discussion20Reference21 TOC o "1-5" h z u Abstract and IntroductionApparatusMediaNumbersMueller Hinton Broth tubes6Mueller Hinton Agar slant tubes6Mueller Hinton Agar stab tubes6Mueller Hinton Agar plae Bacillus subtilis. Hence, Staphylococcus aureus is Gram-positive bacteria. [6]SpeciesFamilyClassGramBacillus subtilisBacillaceaeBacilliPositive (+)Escherichia coliEnterobacteriaceaeGammaproteobacteriaNegative (-)Micrococcus luteusMicrococcaceaeActinobacteriaPositive (+)Serratia marcescensYersiniaceaeGammaproteobacteriaNegative (-)Pseudomonas aeruginosaPseudomonadaceaeGammaproteobacteriaNegative (-)Staphylococcus aureusStaphylococcaceaeBacilliPositive (+)ProceduresProcedure (Aseptic technique and transferring microorganism)Aseptically inoculated one Mueller Hinton Broth tube with Bacillus subtilis, one with Escherichia coli, and one with Staphylococcus aureus.Aseptically inoculate one Mueller Hinton Agar slant tube with Bacillus subtilis, one with Escherichia coli, and one with Staphylococcus aureus.Aseptically inoculate one Mueller Hinton Agar stab tube with Bacillus subtilis, one with Escherichia coli, and one with Staphylococcus aureus.Aseptically inoculate one Mueller Hinton Agar the slant cultures and stab cultures for pigmentation and purity.Mueller Hinton slant tubeCharacteristicsBacillus subtilisEscherichia coliStaphylococcus aureusForm of colonyRhizoidIrregularCircularElevationCrater formConvexConvexMargin (edge)FiliformFiliformEntirePigmentationWhite (Cream)White (Cream)White (Cream)Mueller Hinton agar plateCharacteristicsBacillus subtilisEscherichia coliStaphylococcus aureusForm of colonyRhizoidIrregularCircularElevationCrater formConvexConvexMargin (edge)FiliformFiliformEntirePigmentationWhite (Cream)White (Cream)White (Cream)observedMueller Hinton slant tubeCharacteristicsBacillus subtilisEscherichia coliStaphylococcus aureusForm of colonyCircularCircularCircularElevationFlatFlatFlatMargin (edge)EntireEntireEntirePigmentationWhite (Cream)White (Cream)White (Cream)Mueller Hinton agar plateCharacteristicsBacillus subtilisEscherichia coliStaphylococcus aureusForm of colonyCircularCircularCircularElevationFlatFlatFlatMargin (edge)EntireEntireEntirePigmentmmune system has problem than they have 70 percentage to die by Pseudomonas aeruginosa infection.Culture properties of Pseudomonas aeruginosaWater-soluble pigmentation (Blue Pyocyanin)Yellow fluorescent pigmentation (Fluorsecein)Reddish brown (Pyorubin)Greenish color appears around Pseudomonas aeruginosaTrimethylamine produced smellSerratia marcescens [11]It is a gram-negative Serratia marcescens contained in intestinal bacteria and distinguishes it from other intestinal bacteria because it shows DNase activity. A strain producing a coloring matter insoluble in water in red or pink, and a color-consuming producing bacterium. Although it has been widely distributed in nature and has been referred to as non-pathogenic, it has become important as a cause of the first infectious disease. In most cases, infections in the hospital lead to meningitis and sepsis in infections such as urinary tract, respiratory or wound. It may also cause endocarditis. There are many infections that occur afterure is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.If there are different form of colony, elevation, margin or pigmentation than you may need to concern the contamination of the culture.DiscussionIn the “Optical growth temperature assay” experiment, we have seen that incubating bacteria at temperature 25℃ the Staphylococcus aureus, Pseudomonas aeruginosa, and Serratia marcescens did not grow well as incubating at 37℃. I think Staphylococcus aureus, Pseudomonas aeruginosa, and Serratia marcescens is human pathogen bacteria, therefore those bacteria must grow well in body which 37℃.Serratia marcescens is originally has red pigmentation. However, in our lab it has white and orange pigmentation. Pigmentation of Serratia marcescens relies on the piece of the development medium. The cultures developed ahead glycerol-peptone medium Furthermore on the medium for acetic acid derivation are red Also yellow (yellowish orange), respectively, fAT2
