Protein Purification of Thermostable DNA polymerase

소개글

* Overexpression of Taq polymerase
* Purification of Taq polymerase

목차

1. 실험제목
2. 실험일자
3. 제출지 및 공동 실험자
4. 목적
5. 원리
6. 시약 및 기자재
7. 방법
8. 결과
9. 고찰
10. 참고문헌

본문내용

1. 실험제목
Protein Purification of Thermostable DNA polymerase

2. 실험일자
2006년 5월 1일

4. 목적
* Overexpression of Taq polymerase
* Purification of Taq polymerase

5. 원리
* Taq polymerase
Taq DNA Polymerase was originally isolated from the thermophilic bacterium Thermus aquaticus. The enzyme is thermostable; it replicates DNA at 74°C and remains functional even after incubation at 95°C. It is used, in vitro, to extend a DNA primer using DNA as a template.
Taq DNA Polymerase is a monomeric enzyme with a molecular weight of 94,000Da. The enzyme includes a 5`->3` polymerase activity and a 5`->3` exonuclease activity. Taq DNA Polymerase lacks a 3`->5` exonuclease (proofreading) activity, and will incorporate a deoxynucleotide (often an adenosine) in a template independent manner on the 3` end of the new DNA strand. In in vitro assays, Taq DNA Polymerase misincorporates a base every 104-105 bases.

참고 자료

* http://blog.naver.com/hellopup/20018077047
* http://www.promega.com/faq/taq.html#q01
* David L Nelson, Michael M cox, Lehninger Principles of biochemistry 4th edition, p90~91 , freeman