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[화학생물공정실험]E. coli 와 Yeast의 Cell Culture

"[화학생물공정실험]E. coli 와 Yeast의 Cell Culture"에 대한 내용입니다.
14 페이지
한컴오피스
최초등록일 2023.04.18 최종저작일 2023.04
E. coli yeast Cell Culture 화학생물공정실험
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[화학생물공정실험]E. coli 와 Yeast의 Cell Culture
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  • 미리보기

    목차

    1. 실험 이론 및 원리
    2. 실험 기구 및 시약
    3. 실험 방법
    4. 실험 결과
    5. 토의 사항
    6. 참고 문헌

    본문내용

    1. 실험 이론 및 원리
    가. 실험 요약
    E. coli 와 Yeast Cell Culture의 생식과정을 살펴보고 Spectroscopy를 통해 이를 정량화하여 각 Cell Culture의 지수적 증식을 살펴보았다. 결과적으로 초기의 Lag Phase 를 제외하고는 대체적으로 지수적 증가를 따른다는 것을 확인할 수 있었으며 지수 증가적 모델을 통해 각 Cell Culture의 Specific Growth Rate 과 Doubling Time을 구할 수 있었다.
    한편 계산된 E. coli 와 Yeast 의 Specific Growth Rate과 Doubling Time을 비교함을 통해 본 실험에 어느 정도의 오차가 있었다는 것을 알 수 있었고 이러한 결과를 토대로 실험의 오차를 예측해 보았다. 이외에도 생물학 실험에서 주로 사용되는 멸균법, 실험 시약, Cell Culture 등의 조사를 통해 생물학 실험의 여러 가지 방법을 살펴보았다.

    나. Prokaryote
    원핵생물을 뜻하는 말로써 Carl Woese 에 의해 새로 도입된 분류법의 3가지 Domain - Archaea, Bacteria, Eukaryota - 중 진핵생물인 Eukaryota를 제외한 생물을 지칭하는 말이다. 진핵생물과는 달리 특징적으로 핵막이 없는 원핵생물은 단세포생물일 경우가 많으며 박테리아나 같은 원핵생물 및 주로 무성 생식 방법인 Binary Fission을 통해 번식한다. 이외에도 진핵생물인 Fungi에 속하는 이스트의 몇 가지 종도 Binary Fission을 하며 이를 통해 하나의 모세포가 두 개의 동일한 딸세포로 나뉘게 된다. 본 실험에서는 Escherichia coli, Bacillus subtilis, Yeast 의 Binary Fission을 살펴봄으로 지수적 생식을 확인하고 각 미생물의 Specific Growth Rate과 Doubling Time을 계산하기로 한다.

    참고자료

    · 김영권 외 11명. 미생물학 실험. 고려의학, 2001, pg 115-117
    · 김종협 외 2명. 미생물학 실험서. 대광문화사, 1995 pg 11-16
    · Balasubramanian M, Bi E, Glotzer M (2004). Comparative analysis of cytokinesis in budding yeast, fission yeast and animal cells. Curr Biol 14 (18): R806-18
    · Ingledew WJ, Poole RK (1984). The respiratory chains of Escherichia coli". Microbiol. Rev. 48 (3): 222–71.
    · Black, J.G. (1996). Microbiology. Principles and Applications. 3rd Edition. Prentice Hall. Upper Saddle River, New Jersey. pp. 136-140, 151-153.
    · Tortora, G.J., Funke, B.R., Case, C.L. (1995). Microbiology. An Introduction. 5th Edition. The Benjamin/Cu㎜ings Publishing, Co., Inc., Redwood City, CA, pp. 155-158.
    · Johnson, Ted R. Case, Christine L. (2001). Laboratory Experiments in Microbiology 6th Edition. Benjamin Cu㎜ings.
    · Madigan et. al. (1997) Brock Biology of Microorganisms 8th Edition Prentice Hall International.
    · Lim, Daniel. (1998). Microbiology 2nd Edition. WCB/McGraw-Hill.
    · Shuler, Michael L. Kargi, Fikret. (1992) Bioprocess Engineering Basic Concepts. Prentice Hall.

  • AI와 토픽 톺아보기

    • 1. Prokaryote
      Prokaryotes are single-celled organisms that lack a true nucleus and membrane-bound organelles. They are the simplest and most abundant life forms on Earth, playing crucial roles in various ecosystems. Prokaryotes are divided into two main domains: Archaea and Bacteria. Archaea are often found in extreme environments, while Bacteria are more widely distributed. Prokaryotes have a simpler cellular structure compared to eukaryotes, but they possess remarkable adaptability and metabolic diversity. Understanding prokaryotes is essential for fields such as microbiology, biotechnology, and environmental science, as they have significant impacts on human health, agriculture, and the global ecosystem.
    • 2. Binary Fission
      Binary fission is the primary mode of asexual reproduction in prokaryotes, where a single parent cell divides into two genetically identical daughter cells. This process is a fundamental aspect of prokaryotic biology, enabling rapid population growth and adaptation to diverse environments. During binary fission, the prokaryotic chromosome is replicated, and the two copies are segregated into the daughter cells. The process is tightly regulated and involves various cellular mechanisms to ensure accurate chromosome segregation and cell division. Understanding binary fission is crucial for studying prokaryotic growth, genetics, and the development of antimicrobial strategies. It also provides insights into the evolution and diversification of prokaryotic species.
    • 3. Growth Cycle
      The growth cycle of prokaryotes is a dynamic process that involves several distinct phases, including lag phase, exponential phase, stationary phase, and death phase. During the lag phase, prokaryotic cells adapt to their new environment and prepare for rapid growth. The exponential phase is characterized by an exponential increase in cell numbers, as the cells divide at a constant rate. The stationary phase occurs when the growth rate slows down due to limited resources or the accumulation of waste products. Finally, the death phase is marked by a decline in the number of viable cells. Understanding the growth cycle of prokaryotes is essential for various applications, such as microbial fermentation, bioremediation, and the development of antimicrobial strategies. It also provides insights into the factors that influence prokaryotic population dynamics and their adaptability to changing environmental conditions.
    • 4. Measurement of Cell Growth
      Accurate measurement of prokaryotic cell growth is crucial for various scientific and industrial applications, such as microbiology, biotechnology, and environmental monitoring. Several methods are available for measuring cell growth, including direct cell counting, optical density (OD) measurements, and indirect methods like colony-forming unit (CFU) counts. Direct cell counting involves physically counting the number of cells using a hemocytometer or automated cell counters, providing a direct measure of cell density. Optical density measurements rely on the light-scattering properties of cells, which correlate with cell concentration. Indirect methods, such as CFU counts, involve culturing cells on agar plates and counting the number of colonies formed, which can be used to estimate the number of viable cells. The choice of method depends on the specific application, the type of prokaryotic cells, and the desired level of accuracy and precision. Proper understanding and application of these measurement techniques are essential for studying prokaryotic growth, optimizing fermentation processes, and monitoring environmental microbial populations.
    • 5. Experimental Methods
      Experimental methods in prokaryotic biology are crucial for advancing our understanding of these diverse and ubiquitous organisms. Common experimental approaches include culturing techniques, genetic manipulation, biochemical assays, and microscopy. Culturing methods, such as liquid media and agar plates, allow for the isolation and propagation of specific prokaryotic strains, enabling the study of their physiology, metabolism, and growth characteristics. Genetic manipulation techniques, including transformation, transduction, and conjugation, facilitate the introduction of genetic modifications and the investigation of gene function. Biochemical assays, such as enzyme activity measurements and metabolite quantification, provide insights into the molecular mechanisms underlying prokaryotic processes. Microscopy techniques, including light microscopy and electron microscopy, enable the visualization of prokaryotic cell structure, morphology, and interactions. The careful design and execution of these experimental methods, combined with rigorous data analysis, are essential for generating reliable and reproducible findings in prokaryotic biology, which can then be applied to diverse fields, from biotechnology to environmental microbiology.
    • 6. Error Analysis
      Error analysis is a critical component of experimental methods in prokaryotic biology, as it helps researchers understand the reliability and limitations of their data. Potential sources of error in prokaryotic studies include sampling errors, measurement errors, and experimental design flaws. Sampling errors can arise from the heterogeneity of microbial populations or the uneven distribution of cells in a sample. Measurement errors can be introduced by the use of imprecise instruments or the inherent variability of biological systems. Experimental design flaws, such as inadequate controls or confounding factors, can also contribute to errors. Rigorous error analysis, including the calculation of standard deviations, confidence intervals, and statistical significance, allows researchers to quantify the uncertainty in their results and make informed decisions about the validity and interpretation of their findings. By incorporating error analysis into their experimental protocols, prokaryotic biologists can improve the reliability of their research, identify sources of variability, and develop more robust experimental designs. This, in turn, enhances the overall quality and reproducibility of studies in the field of prokaryotic biology.
    • 7. Sterilization Methods
      Sterilization methods are essential in prokaryotic biology to ensure the integrity and reliability of experimental results by eliminating unwanted microbial contamination. Common sterilization techniques include autoclaving, filtration, and the use of chemical disinfectants. Autoclaving, which involves exposing materials to high-pressure steam, is a highly effective method for killing a wide range of prokaryotic cells and spores. Filtration, using membranes with pore sizes small enough to retain microorganisms, is useful for sterilizing heat-sensitive solutions. Chemical disinfectants, such as alcohols, bleach, and quaternary ammonium compounds, can also be employed to inactivate prokaryotic cells on surfaces and in liquids. The choice of sterilization method depends on the nature of the materials, the types of prokaryotic organisms present, and the specific experimental requirements. Proper implementation of sterilization protocols is crucial to maintain the integrity of cultures, reagents, and equipment, ensuring the reliability and reproducibility of prokaryotic research. Understanding the principles and limitations of various sterilization methods is essential for researchers working in the field of prokaryotic biology.
    • 8. Measurement of Cell Growth
      Accurate measurement of prokaryotic cell growth is crucial for various scientific and industrial applications, such as microbiology, biotechnology, and environmental monitoring. Several methods are available for measuring cell growth, including direct cell counting, optical density (OD) measurements, and indirect methods like colony-forming unit (CFU) counts. Direct cell counting involves physically counting the number of cells using a hemocytometer or automated cell counters, providing a direct measure of cell density. Optical density measurements rely on the light-scattering properties of cells, which correlate with cell concentration. Indirect methods, such as CFU counts, involve culturing cells on agar plates and counting the number of colonies formed, which can be used to estimate the number of viable cells. The choice of method depends on the specific application, the type of prokaryotic cells, and the desired level of accuracy and precision. Proper understanding and application of these measurement techniques are essential for studying prokaryotic growth, optimizing fermentation processes, and monitoring environmental microbial populations.

  • 자료후기

      Ai 리뷰
      실험 결과는 전체적으로 지수적 증가 모델을 잘 따르고 있으며, 계산된 Specific Growth Rate과 Doubling Time을 통해 E. coli와 Yeast의 증식 속도 차이를 확인할 수 있었다.

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