Epigallocatechin Gallate (EGCG)의 포름알데히드에 의한폐 대식세포 사멸 차단 효과 (The Protective Effect of Epigallocatechin Gallate (EGCG) against Formaldehyde-induced Cell Death in Alveolar Macrophage)

서지정보

· 발행기관 : 한국차학회
· 수록지 정보 : 한국차학회지 / 21권 / 2호 / 54 ~ 59페이지
· 저자명 : 오영준, 최주희, 윤효인, 박수현

초록

본 연구에서는 폐 대식세포인 Raw 264.7 세포를 이용하여 녹차 주요 성분인 EGCG 처리 하였을 때 FA에 의해서야기되는 세포 세포사멸 증가, 산화성 스트레스증가 작용이 차단되는 것을 확인하였다. 아울러 FA에 의한 세포 사멸 촉진 단백질 증가 작용 및 세포 사멸 억제 단백질 감소작용은 녹차 주요 성분인 EGCGC 처리 하였을 때도 차단되었다. 결론적으로 녹차의 EGCG를 주성분으로 하는catechin 성분이 FA에 의한 폐 대식세포의 산화성 스트레스 증가 작용 억제를 통하여 폐 대식 세포사멸을 예방하는것으로 나타났다.

영어초록

The consumption of green tea (GT) is linked to a decrease in the onset of diverse diseases in humans.
Epigallocatechin-3-gallate (EGCG) is the major catechin of green tea. Formaldehyde (FA) is an important irritant compound in pesticides to induce asthma and allergy in the respiratory system. Alveolar macrophages are also a pivotal cell type in the immune response of the respiratory system. On the other hand, the effects of EGCG on FA-induced cell death has not been elucidated. Therefore, this study examined the preventive effects of EGCG against FA-induced cell death in Raw 264.7 cells, an alveolar macrophage cell line. In the present study, FA induced a decrease in the cell viability and GSH contents, and stimulated lipid peroxide [LPO] formation. A treatment with green tea extracts (GTE) and EGCG (> 1 μM) prevented FA-induced cell death, lipid peroxide formation, and GSH contents in Raw 264.7 cells.
The treatment with GTE and EGCG also prevented the FA-induced increase of cleaved caspase-3 and Bax, pro-apoptotic proteins, and a decrease in Bcl-2, an anti-apoptotic protein in Raw 264.7 cells. Hence, it is expected that EGCG from green tea offers protection against FA-induced cell death and its main action is mediated by a decrease in oxidative stress. In conclusion, green tea extracts prevented FA-induced cell death by decreasing the levels of oxidative stress in Raw 264.7 cells.

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