북방 전복 (Haliotis discus hannai) 에서 분리한 항균 펩타이드 LGBP의 생체 내 독성 평가 (In vivo toxicity assessment of novel antimicrobial peptide derived from lipopolysaccharide- and ß-1, 3-glucan-binding protein (LGBP) of the pacific abalone (Haliotis discus hannai))

서지정보

· 발행기관 : 한국패류학회
· 수록지 정보 : The Korean Journal of Malacology / 36권 / 1호 / 23 ~ 34페이지
· 저자명 : 신은하, 박은희, 노은수, 김영옥, 김동균, 공희정, 박중연, 남보혜

초록

Antimicrobial peptides (AMPs) constitute important components of the innate immunity, protecting the host against infections. A synthetic antimicrobial peptide was identified as a possible candidate for the development of a new antibacterial drug. We previously developed novel AMPs derived from a lipopolysaccharide- and ß-1, 3-glucan-binding protein (LGBP) of the pacific abalone Haliotis discus hannai (HDH) particularly showing antimicrobial against bacteria and fungi and antitumor activities against several cancer cells.To evaluate in-vivo toxicity of AMPs is an essential step to ensure it safe use in drugs and foods. In the present study, we have investigated to evaluate in-vivo toxicity of HDH-LGBP in mouse when orally administered to both sexes of ICR mice (10 males and 10 females in each group) at dose of 50, 100 and 200 mg/kg/day for 14 days. Individual food/ water consumption and body weight changes were measured daily. The group treated with HDH-LGBP didn’t show any histological change in the kidney and liver. Statistically significant decreases of food consumption, serum creatinine and platelet were found in 200 mg HDH-LGBP-treated groups when compared to their respective controls. It was considered to be caused by stress and peptide gelation. Male and female mice did not show effects body weight, food consumption, organ weights, hematology, serum chemistry, urinalysis or microscopic pathology. Therefore, the no-observed-adverse-effect level (NOAEL) for HDH-LGBP was concluded to be a 200 mg/kg/day. These results corroborate the potential of this novel AMP (HDH-LGBP) as an alternative food preservative or multifunctional drug.

영어초록

Antimicrobial peptides (AMPs) constitute important components of the innate immunity, protecting the host against infections. A synthetic antimicrobial peptide was identified as a possible candidate for the development of a new antibacterial drug. We previously developed novel AMPs derived from a lipopolysaccharide- and ß-1, 3-glucan-binding protein (LGBP) of the pacific abalone Haliotis discus hannai (HDH) particularly showing antimicrobial against bacteria and fungi and antitumor activities against several cancer cells.To evaluate in-vivo toxicity of AMPs is an essential step to ensure it safe use in drugs and foods. In the present study, we have investigated to evaluate in-vivo toxicity of HDH-LGBP in mouse when orally administered to both sexes of ICR mice (10 males and 10 females in each group) at dose of 50, 100 and 200 mg/kg/day for 14 days. Individual food/ water consumption and body weight changes were measured daily. The group treated with HDH-LGBP didn’t show any histological change in the kidney and liver. Statistically significant decreases of food consumption, serum creatinine and platelet were found in 200 mg HDH-LGBP-treated groups when compared to their respective controls. It was considered to be caused by stress and peptide gelation. Male and female mice did not show effects body weight, food consumption, organ weights, hematology, serum chemistry, urinalysis or microscopic pathology. Therefore, the no-observed-adverse-effect level (NOAEL) for HDH-LGBP was concluded to be a 200 mg/kg/day. These results corroborate the potential of this novel AMP (HDH-LGBP) as an alternative food preservative or multifunctional drug.

참고자료

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