검정콩 종피 함유 안토시아닌의 최적 추출조건 (Optimal Extraction Condition of Anthocyanins in Soybean (Glycine max) with Black Seed Coats)

서지정보

· 발행기관 : 한국작물학회
· 수록지 정보 : 한국작물학회지 / 53권 / 1호 / 110 ~ 117페이지
· 저자명 : 정명근, 황영선, 이현진, 최수산나, 임정대, 강성택, 한원영, 백인열, 김현경

초록

Soybeans (Glycine max) with a black seed
coat have been widely utilized as food source and as a
medicinal herbs in Korea. The pigmentation in the seed
coat of black soybean is due to accumulate anthocyanins
in the epidermis palisade layer. The anthocyanin content
and composition of the black soybean seed coat are considered
as a standard of quality evaluation of black soybean.
The main objective of this study was to investigate
the optimal condition for an extraction method of anthocyanins
and compare anthocyanin quantity and composition
within black soybean varieties and germplasms. In the test
of extraction solvent, absorbance at 530 nm and Hunter’s
a value were increased as increasing the concentration of
MeOH, but Hunter’s L and b values were the exact opposite
of absorbance and Hunter’s a values. There was no
significant difference for anthocyanin contents from 1%
HCl - H₂O to 1% HCl - 80% MeOH. In the aspects of anthocyanin
contents and HPLC peak resolution, 1% HCl - 20%
MeOH extraction solution was the most suitable solvent.
Among the 5 kinds of extraction method using 1% HCl -
20% MeOH solution, the anthocyanin contents of room
temperature extraction at 72 h was the highest among the
methods. High extraction temperature, sonication and reflux
method influenced on the decrease of anthocyanin contents
because of breakdown of anthocyanins. There was no significant
difference for extraction time between 12 h and 24
h. However, the optimal extraction condition were at room
temperature for 12 h. The anthocyanin contents in seed
coats of black soybean were determined on the basis of
HPLC peak area at 530 nm. Ten black soybean varieties and
germplasms were tested with optimal conditions founded in
this study. On the basis of antocyanin component, these
can be classified into three groups; C3G, C3G + D3G and
C3G + D3G + Pt3G. The total anthocyanin content in seed
coats ranged from 1.58 to 10.62 mg/g of seed. The total
anthocyanin content of the variety “Geomjeongol” was about
7 times higher than that of variety “Heugchong”. Information
for extraction method and diversity in antocyanin
of soybean seed coats can be used for future research for
germplasm evaluation and development of high quality black
soybean varieties.

영어초록

Soybeans (Glycine max) with a black seed
coat have been widely utilized as food source and as a
medicinal herbs in Korea. The pigmentation in the seed
coat of black soybean is due to accumulate anthocyanins
in the epidermis palisade layer. The anthocyanin content
and composition of the black soybean seed coat are considered
as a standard of quality evaluation of black soybean.
The main objective of this study was to investigate
the optimal condition for an extraction method of anthocyanins
and compare anthocyanin quantity and composition
within black soybean varieties and germplasms. In the test
of extraction solvent, absorbance at 530 nm and Hunter’s
a value were increased as increasing the concentration of
MeOH, but Hunter’s L and b values were the exact opposite
of absorbance and Hunter’s a values. There was no
significant difference for anthocyanin contents from 1%
HCl - H₂O to 1% HCl - 80% MeOH. In the aspects of anthocyanin
contents and HPLC peak resolution, 1% HCl - 20%
MeOH extraction solution was the most suitable solvent.
Among the 5 kinds of extraction method using 1% HCl -
20% MeOH solution, the anthocyanin contents of room
temperature extraction at 72 h was the highest among the
methods. High extraction temperature, sonication and reflux
method influenced on the decrease of anthocyanin contents
because of breakdown of anthocyanins. There was no significant
difference for extraction time between 12 h and 24
h. However, the optimal extraction condition were at room
temperature for 12 h. The anthocyanin contents in seed
coats of black soybean were determined on the basis of
HPLC peak area at 530 nm. Ten black soybean varieties and
germplasms were tested with optimal conditions founded in
this study. On the basis of antocyanin component, these
can be classified into three groups; C3G, C3G + D3G and
C3G + D3G + Pt3G. The total anthocyanin content in seed
coats ranged from 1.58 to 10.62 mg/g of seed. The total
anthocyanin content of the variety “Geomjeongol” was about
7 times higher than that of variety “Heugchong”. Information
for extraction method and diversity in antocyanin
of soybean seed coats can be used for future research for
germplasm evaluation and development of high quality black
soybean varieties.

참고자료

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