감마선 및 화학적 돌연변이원 처리가 스테비아(Stevia rebaudiana Bert.)의 종자 발아 및 초기 생장에 미치는 영향 (Effects of Gamma-ray and Chemical Mutagens on the Germination and Seedling Growth in Stevia rebaudiana Bert.)

서지정보

· 발행기관 : (사)한국방사선산업학회
· 수록지 정보 : 방사선산업학회지 / 6권 / 2호 / 189 ~ 197페이지
· 저자명 : 윤태영, 김이엽, 김영호, 최진수, 현경섭, 성윤희, 조한직, 김동섭, 강시용, 고정애

초록

This study was carried out to develop the improved useful mutants for yield orcomposition of stevia plants using the gamma ray or chemical mutagens treatments. The seeds ofstevia ‘Suwon No. 11’ were irradiated up to 400 Gy of gamma ray. Chemical mutagens weretreated on the seeds of the ‘Suwon No. 11’ using 0.07% colchicine, 10 mM sodium azide, or 10mM NMU for various durations. The germination rate, and shoot and root growth of seedlingwere estimated at 30 days after gamma ray irradiation or chemical mutagen treatment, and theplant height, the number of branches, and leaf length and width were examined at 3 months aftermutagenesis treatments. In the case of gamma ray treatments, the germination rate and earlystagegrowth were decreased as the increase of radiation dose, and the 50% lethal dose was foundto be 200 Gy. the plant height was decreased as the increase of radiation dose, while the numberof branches per plant and leaf length were increased. Leaf shape was modified to the relativelylonger one compared to the control, which was identified more apparently at the treatments ofhigher than 150 Gy. In the treatment of chemical mutagens, the rate of germination and survivalwere decreased as the increase of incubation time. The 50% lethal dose for germination rate wereidentified as the conditions of the 15 hours incubation in 0.07% colchicine, the 4 hrs in 10 mMsodium azide, and the 2 hrs in 10 mM NMU, in the three chemical mutagens treatments. Chemicalmutagens had no influence on shoot growth, while root growth was increased, especially as theincubation time was extended. The highest root growth occurred in the NMU treatment at 6 hrsincubation time. The plant height was decreased as the increase of incubation time in the chemicalmutagens treatments. Among the chemical mutagens, NMU was the most effective to induce themutants with long-shaped or the least lobed leaves.

영어초록

This study was carried out to develop the improved useful mutants for yield orcomposition of stevia plants using the gamma ray or chemical mutagens treatments. The seeds ofstevia ‘Suwon No. 11’ were irradiated up to 400 Gy of gamma ray. Chemical mutagens weretreated on the seeds of the ‘Suwon No. 11’ using 0.07% colchicine, 10 mM sodium azide, or 10mM NMU for various durations. The germination rate, and shoot and root growth of seedlingwere estimated at 30 days after gamma ray irradiation or chemical mutagen treatment, and theplant height, the number of branches, and leaf length and width were examined at 3 months aftermutagenesis treatments. In the case of gamma ray treatments, the germination rate and earlystagegrowth were decreased as the increase of radiation dose, and the 50% lethal dose was foundto be 200 Gy. the plant height was decreased as the increase of radiation dose, while the numberof branches per plant and leaf length were increased. Leaf shape was modified to the relativelylonger one compared to the control, which was identified more apparently at the treatments ofhigher than 150 Gy. In the treatment of chemical mutagens, the rate of germination and survivalwere decreased as the increase of incubation time. The 50% lethal dose for germination rate wereidentified as the conditions of the 15 hours incubation in 0.07% colchicine, the 4 hrs in 10 mMsodium azide, and the 2 hrs in 10 mM NMU, in the three chemical mutagens treatments. Chemicalmutagens had no influence on shoot growth, while root growth was increased, especially as theincubation time was extended. The highest root growth occurred in the NMU treatment at 6 hrsincubation time. The plant height was decreased as the increase of incubation time in the chemicalmutagens treatments. Among the chemical mutagens, NMU was the most effective to induce themutants with long-shaped or the least lobed leaves.

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