효소처리에 의한 참당귀 다당체 분리 연구 (A Study on Isolation of Polysaccharides from Angelica gigas Nakai by Enzyme Treatments)

서지정보

· 발행기관 : 한국산업식품공학회
· 수록지 정보 : 산업식품공학 / 18권 / 4호 / 406 ~ 412페이지
· 저자명 : 이희정, 정헌상, 박춘근, 이정훈, 박충범, 김종태, 최애진

초록

This study conducted enzyme treatments to maximize the extraction of polysaccharide components in Angelica gigas Nakai (A. gigas). An extraction method of polysaccharides was established by using different enzyme treatments for the degradation of plant cell walls. Total carbohydrates and removed starch of A. gigas increased after enzyme treatments such as Viscozyme (V), Alcalase (A), Flavorzyme (F) and Termamyl 120L (T). The yield and total carbohydrates of VAFT were 12.20% and 76.80%, respectively; an increase of 1.4 times that of T and AFT and showed a significant difference (p<0.05). Removed starch content also increased by more than three times the control in water-extractable polysaccharides. Non-starch polysaccharide content of VAFT was 22.58%; it increased about 1.7 times that of the control (12.84%) and it was the highest compared to T and AFT. Arabinose and galactose, which were the main components of A. gigas polysaccharides, increased from 1.5 to 2.2 times the control in the enzyme treatment groups. The high molecular weight fraction I of the control, depending on enzyme treatment conditions, was about 491,282 Da. It degraded into a low molecular weight fraction ranged from 282,459 to 16,362 Da. The molecular weight of polysaccharides treated by the VAFT decreased to an average of 13,000 Da. So, VAFT was selected as the optimizing condition for extracting water extractable polysaccharides from A. gigas. As a result of this study, it is expected that A. gigas will be widely used for industrial materials and useful to develop and research materials of different medicinal plants which are not yet known.

영어초록

This study conducted enzyme treatments to maximize the extraction of polysaccharide components in Angelica gigas Nakai (A. gigas). An extraction method of polysaccharides was established by using different enzyme treatments for the degradation of plant cell walls. Total carbohydrates and removed starch of A. gigas increased after enzyme treatments such as Viscozyme (V), Alcalase (A), Flavorzyme (F) and Termamyl 120L (T). The yield and total carbohydrates of VAFT were 12.20% and 76.80%, respectively; an increase of 1.4 times that of T and AFT and showed a significant difference (p<0.05). Removed starch content also increased by more than three times the control in water-extractable polysaccharides. Non-starch polysaccharide content of VAFT was 22.58%; it increased about 1.7 times that of the control (12.84%) and it was the highest compared to T and AFT. Arabinose and galactose, which were the main components of A. gigas polysaccharides, increased from 1.5 to 2.2 times the control in the enzyme treatment groups. The high molecular weight fraction I of the control, depending on enzyme treatment conditions, was about 491,282 Da. It degraded into a low molecular weight fraction ranged from 282,459 to 16,362 Da. The molecular weight of polysaccharides treated by the VAFT decreased to an average of 13,000 Da. So, VAFT was selected as the optimizing condition for extracting water extractable polysaccharides from A. gigas. As a result of this study, it is expected that A. gigas will be widely used for industrial materials and useful to develop and research materials of different medicinal plants which are not yet known.

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