vWF형질전환돼지에서 재조합체 단백질의 발현 양상 분석 (Analysis of the Expression Pattern of Recombinant Proteins in vWF Transgenic Pigs)

서지정보

· 발행기관 : 사단법인 한국동물생명공학회
· 수록지 정보 : Reproductive & Developmental biology / 35권 / 3호 / 417 ~ 421페이지
· 저자명 : 채인순, 황환진, 정희경, 이현기, 김성우, 정학재, 이휘철, 양병철, 박춘근, 박진기, 김경운

초록

We generated transgenic pig that produced recombinant human von Willebrand factor(rhvWF) in its milk using microinjection with bovine alpha S1-casein gene promoter controlled hvWF cDNA expression vector. Transgene was stably inserted into host genome from F0 to F3 generation of transgenic pig. This study is to analysis of expression patterns of hvWF in three lines. According to Western blotting and ELISA, the rhvWF expression of V3 line showed the highest level among three lines. Real time PCR and Southern blotting data showed that V1 had the largest copy number. To identify chromosome integration site, FISH and karyotyping were performed. Consequently, the transgene was located in chromosome 15q13.1 of V1, in chromosome 11p11.2 of V2, and in chromosome 4p13 of V3. Therefore,these results indicate that the expression level of vWF in transgenic pig was involved in the integration locus of chromosome.

영어초록

We generated transgenic pig that produced recombinant human von Willebrand factor(rhvWF) in its milk using microinjection with bovine alpha S1-casein gene promoter controlled hvWF cDNA expression vector. Transgene was stably inserted into host genome from F0 to F3 generation of transgenic pig. This study is to analysis of expression patterns of hvWF in three lines. According to Western blotting and ELISA, the rhvWF expression of V3 line showed the highest level among three lines. Real time PCR and Southern blotting data showed that V1 had the largest copy number. To identify chromosome integration site, FISH and karyotyping were performed. Consequently, the transgene was located in chromosome 15q13.1 of V1, in chromosome 11p11.2 of V2, and in chromosome 4p13 of V3. Therefore,these results indicate that the expression level of vWF in transgenic pig was involved in the integration locus of chromosome.

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