냉동보관 제대혈 신생혈관 유도성세포배양과 허혈성 하지 쥐모델에서의 치료효과연구 (Cryopreserved Human Umbilical Cord Blood-derived Angiogenic Cells Therapy for Ischemic hindlimb in Mice)

서지정보

· 발행기관 : 한국조직공학과 재생의학회
· 수록지 정보 : 조직공학과 재생의학 / 6권 / 4호 / 805 ~ 810페이지
· 저자명 : 김성환, 변기현, 손영덕

초록

Currently, human bone marrow was thought to be the main source of stem cells. However, bone marrow derived stem cell is a limited source of cells for allograft transplantation, because it is not easy to collect bone marrow and they also have the possibility of viral infection. Therefore, as an alternative stem cell source, human umbilical cord blood(hUCB) could overcome the limitations of bone marrow derived stem cells. However, it has been
difficult to isolate mesenchymal stem cells(MSCs) from cryopreserved hUCB due to the containment of rare populations of mesenchymal progenitor cells(MPCs). We tried to establish the culture system for angiogenic cells isolation
from cryopreserved hUCB. As a result, we successfully could culture angiogenic cells that have endothelial specific surface markers and the capacity for differentiation potential into endothelial cells. In addition, transplantation of these cells into ischemic hindlimb mice showed that increased blood flow and higher limb salvage rate in the hindlimb ischemia. Immunohistochemistry results also demonstrated that hUCB derived cells are detected in the
arterial walls of the ischemic hindlimb. Therefore, it is suggested that transplantation of cultured angiogenic cells derived from cryopreserved hUCB may be a novel and useful therapeutic armament for similar ischemic diseases.

영어초록

Currently, human bone marrow was thought to be the main source of stem cells. However, bone marrow derived stem cell is a limited source of cells for allograft transplantation, because it is not easy to collect bone marrow and they also have the possibility of viral infection. Therefore, as an alternative stem cell source, human umbilical cord blood(hUCB) could overcome the limitations of bone marrow derived stem cells. However, it has been
difficult to isolate mesenchymal stem cells(MSCs) from cryopreserved hUCB due to the containment of rare populations of mesenchymal progenitor cells(MPCs). We tried to establish the culture system for angiogenic cells isolation
from cryopreserved hUCB. As a result, we successfully could culture angiogenic cells that have endothelial specific surface markers and the capacity for differentiation potential into endothelial cells. In addition, transplantation of these cells into ischemic hindlimb mice showed that increased blood flow and higher limb salvage rate in the hindlimb ischemia. Immunohistochemistry results also demonstrated that hUCB derived cells are detected in the
arterial walls of the ischemic hindlimb. Therefore, it is suggested that transplantation of cultured angiogenic cells derived from cryopreserved hUCB may be a novel and useful therapeutic armament for similar ischemic diseases.

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