Biotic 물질이 콩 발아 중 Isoflavone 함량에 미치는 영향 (Effect of Biotic Substances on Isoflavone Content in Soybean Germination)

서지정보

· 발행기관 : 한국작물학회
· 수록지 정보 : 한국작물학회지 / 65권 / 2호 / 84 ~ 92페이지
· 저자명 : 김서영, 송영호, 이유정, 김홍식, 김용호

초록

콩 발아기간 중 몇 가지 면역활성제(자스몬산, 키토산,베타글루칸 및 게르마늄)와 녹차 및 인삼 추출물이 이소플라본 함량 증대에 미치는 영향에 대하여 알아보았다.
1. 대부분의 처리에서 발아시간이 경과함에 따라 이소플라본 함량이 높아졌는데 면역활성제 중에서는 키토산 처리가 이소플라본 함량 증대에 가장 큰 효과가 있었으며약초추출물 처리는 효과가 미미하였다.
2. 품종별 이소플라본 함량은 모든 처리에서 대풍2호>우람>풍산나물콩의 순서로 높았으며 이소플라본 함량은품종과 biotic 물질 처리간 상호작용도 영향을 미치는 것으로 판단된다.
3. 개별 이소플라본 종류별 함량을 분석한 결과 malonyl�glucoside>glucoside>aglycone>acetyl-glucoside 순서로함량이 높았으며 발아가 진행되면서 aglycone 함량이증가하는 경향이었다.
따라서 콩 발아기간 중 biotic 물질 처리는 어느 정도 이소플라본 함량을 증대시키며 품종과 물질처리간 상호작용효과도 있음을 확인할 수 있었다.

영어초록

For humans, soybean and soybean products are the main dietary sources of isoflvones, which are polyphenoliccompounds that represent one of the most common categories of phytoestrogens. The objective of this study was to determineisoflavone concentrations in soybean cultivars during germination when treated with some biotic substances. Three soybeancultivars were germinated in replicated trials in 2018/2019 and their individual and total isoflavone concentrations weredetermined using HPLC (High-Performance Liquid Chromatography). Significant differences were observed in total isoflavonecontent were observed among cultivars regardless of years and treatments. ‘Daepung2-ho’ and ‘Uram’ had significantly highertotal isoflavones than ‘Pungsannaul-kong’. Differences among treatments were also significant for total isoflavone content. In2018, with chitosan treatment, total isoflavone concentration ranged from 551.15 to 7584.07 μg g-1, with an average of 2972.64μg g-1 across cultivars. In 2019, there was no significant difference among treatments in total isoflavone content. Regardingindividual isoflavone concentrations, the malonyl-glucoside groups accounted for over 85% of the total isoflavone content, whichis indicated that these groups play an important role with regard to isoflavone components in soybean seeds. The individualproportions in the total concentrations of isoflavones varied according to germination period and seed tissues. Glucosides andmalonyl-glucosides showed differences in concentrations among seed tissues, aglycones were further accumulated as germinationperiod was progressed. This study suggests that biotic substances have an impact on seed isoflavone content during germination.
However, cultivars with consistently high or low isoflavone concentrations across biotic substance treatments were identifieddesspite differences in germination period and seed tissues, demonstrating that the genetic factor plays the most important role inisoflavone accumulation.

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