左歸丸이 산화적 손상, 염증 및 골절유합 관련 인자에 미치는 영향 (Effects of Joaguihwan (JGH, 左歸丸) Extract on Changes of Anti-oxidation, Anti-inflammatory in RAW 264.7 Cells and on Factors Related with Bone Metabolism in Skull Fractured Rat)

서지정보

· 발행기관 : 한방재활의학과학회
· 수록지 정보 : 한방재활의학과학회지 / 26권 / 3호 / 31 ~ 49페이지
· 저자명 : 이옥진, 오민석

초록

ObjectivesThe study was designed to evaluate the healing effects of Joaguihwan (JGH) extract on Anti-oxidation, Anti-inflammatory in RAW 264.7 Cells and factors related with bone metabolism in skull fractured Rat.MethodsThe fracture healing effect of JGH was measured by scavenging activities of1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and nitric oxide (NO) in RAW 264.7 cells. The inhibitory effect against the pro-duction of inflammatory mediators including interleukin-1β (IL-1β), interleukin-6 (IL-6), tu-mor necosis factors-α (TNF-α) expression was inhibited in RAW 264.7 cells was ex-perimented using JGH. The effects of JGH on healing fractured rats was measured by os-teocalcin, calcitonin, CTXII, TGF-β, BMP-2, Insulin, ALP in the serum. and was checked every 3 weeks from 0 week to 6week using x-ray.Results1. DPPH free radica and ABTS scavenging activity of JGH were increased ac-cording to concentration of JGH in RAW 264.7 Cells. 2. In the experiment, NO, IL-1β, IL-6, TNF-α all showed decrease, in general. Especially NO and IL-1β showed significantly de-crease at a concentration of 10, 100 (μg/ml). 3. In the production of osteocalcin in the se-rum, JGH 200, 400 mg/kg experimental group showed significant increased effect at 2 weeks. 4. In the production of calcitonin in the serum. JGH 200 mg/kg experimental group showed significant increased effect at 4, 6 weeks. JGH 400 mg/kg experimental group showed significant increased effect at 2, 4, 6 weeks. 5. In the production of CTX, TGF-β, BMP-2 in the serum, experimental group showed increased effect. but no significant effect. 6. In the production of insulin in the serum. JGH 200, 400 mg/kg experimental group showed significant decrease effect at 2, 4, 6 weeks. 7. In the production of ALP in the serum. JGH 200 mg/kg experimental group showed significant increased effect at 2, 4, 6 weeks. JGH 400 mg/kg experimental group showed significant increased effect at 4, 6 weeks. 8. In the change of X-ray, the experimental group showed better healing effects on skull fractured rats than control group.ConclusionsFrom above results, JGH showed healing effect on Anti-oxidation, Anti-in-flammatory in RAW 264.7 Cells, factors related with bone metabolism in the serum of skull fractured rat and x-ray, which is expected to be applied in clinics. (J Korean Med Rehabil 2016;26(3):31-49)

영어초록

ObjectivesThe study was designed to evaluate the healing effects of Joaguihwan (JGH) extract on Anti-oxidation, Anti-inflammatory in RAW 264.7 Cells and factors related with bone metabolism in skull fractured Rat.MethodsThe fracture healing effect of JGH was measured by scavenging activities of1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and nitric oxide (NO) in RAW 264.7 cells. The inhibitory effect against the pro-duction of inflammatory mediators including interleukin-1β (IL-1β), interleukin-6 (IL-6), tu-mor necosis factors-α (TNF-α) expression was inhibited in RAW 264.7 cells was ex-perimented using JGH. The effects of JGH on healing fractured rats was measured by os-teocalcin, calcitonin, CTXII, TGF-β, BMP-2, Insulin, ALP in the serum. and was checked every 3 weeks from 0 week to 6week using x-ray.Results1. DPPH free radica and ABTS scavenging activity of JGH were increased ac-cording to concentration of JGH in RAW 264.7 Cells. 2. In the experiment, NO, IL-1β, IL-6, TNF-α all showed decrease, in general. Especially NO and IL-1β showed significantly de-crease at a concentration of 10, 100 (μg/ml). 3. In the production of osteocalcin in the se-rum, JGH 200, 400 mg/kg experimental group showed significant increased effect at 2 weeks. 4. In the production of calcitonin in the serum. JGH 200 mg/kg experimental group showed significant increased effect at 4, 6 weeks. JGH 400 mg/kg experimental group showed significant increased effect at 2, 4, 6 weeks. 5. In the production of CTX, TGF-β, BMP-2 in the serum, experimental group showed increased effect. but no significant effect. 6. In the production of insulin in the serum. JGH 200, 400 mg/kg experimental group showed significant decrease effect at 2, 4, 6 weeks. 7. In the production of ALP in the serum. JGH 200 mg/kg experimental group showed significant increased effect at 2, 4, 6 weeks. JGH 400 mg/kg experimental group showed significant increased effect at 4, 6 weeks. 8. In the change of X-ray, the experimental group showed better healing effects on skull fractured rats than control group.ConclusionsFrom above results, JGH showed healing effect on Anti-oxidation, Anti-in-flammatory in RAW 264.7 Cells, factors related with bone metabolism in the serum of skull fractured rat and x-ray, which is expected to be applied in clinics. (J Korean Med Rehabil 2016;26(3):31-49)

참고자료

· 없음