Ursolic acid의 악성 흑색종 세포주 A375SM과 A375P에서의 항암효능 (Anti-Cancer Effect of Ursolic Acid in Melanoma Cell A375SM and A375P)

서지정보

· 발행기관 : 한국식품위생안전성학회
· 수록지 정보 : 한국식품위생안전성학회지 / 34권 / 2호 / 183 ~ 190페이지
· 저자명 : 우중석, 김나원, 이진규, 김재혁, 임다영, 강신우, 김성현, 유은선, 이재한, 한소희, 박영석, 김병수, 김상기, 박병권, 정지윤

초록

Ursolic acid is recognized for various effects such as anti-cancer, antioxidant, and anti-inflamma- tory activity. In this study, we confirmed the anti-cancer effect of ursolic acid on human melanoma cancer cells, A375SM and A375P. Survival rate of the melanoma cells was confirmed by MTT assay and the proliferation rate was confirmed by wound healing assay. The rate of apoptotic bodies was confirmed by DAPI staining, and apoptosis rate was confirmed by flow cytometry. The induction of apoptosis protein was examined by western blotting according to the concentration of ursolic acid in melanoma cells. The survival and proliferation rates of melanoma cells were decreased according to the treatment concentrations of ursolic acid. DAPI staining showed that chromosomal conden- sation of melanoma cells was increased with increasing concentrations of ursolic acid, and increased apoptosis rate of melanoma cells by ursolic acid was confirmed by flow cytometry. We also confirmed by western blotting that cleaved- PARP and Bax were increased and Bcl-2 was decreased at 12 µM concentration of uricolic acid in melanoma cells.
This study was carried out at low concentrations of ursolic acid, 0 to 20 µM, and analyzed 24 h after treatment. As a result of this study, it is thought that ursolic acid has the anti-cancer effect through the regulation of apoptosis-related proteins in melanoma cells A375SM and A375P.

영어초록

Ursolic acid is recognized for various effects such as anti-cancer, antioxidant, and anti-inflamma- tory activity. In this study, we confirmed the anti-cancer effect of ursolic acid on human melanoma cancer cells, A375SM and A375P. Survival rate of the melanoma cells was confirmed by MTT assay and the proliferation rate was confirmed by wound healing assay. The rate of apoptotic bodies was confirmed by DAPI staining, and apoptosis rate was confirmed by flow cytometry. The induction of apoptosis protein was examined by western blotting according to the concentration of ursolic acid in melanoma cells. The survival and proliferation rates of melanoma cells were decreased according to the treatment concentrations of ursolic acid. DAPI staining showed that chromosomal conden- sation of melanoma cells was increased with increasing concentrations of ursolic acid, and increased apoptosis rate of melanoma cells by ursolic acid was confirmed by flow cytometry. We also confirmed by western blotting that cleaved- PARP and Bax were increased and Bcl-2 was decreased at 12 µM concentration of uricolic acid in melanoma cells.
This study was carried out at low concentrations of ursolic acid, 0 to 20 µM, and analyzed 24 h after treatment. As a result of this study, it is thought that ursolic acid has the anti-cancer effect through the regulation of apoptosis-related proteins in melanoma cells A375SM and A375P.

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