가교결합된 콜라젠 기질의 물리적 특성과 체내 염증반응의 상관관계 (Correlation of In Vitro Physical Properties and In Vivo Inflammatory Reaction with Cross-linked Collagen Matrix)

서지정보

· 발행기관 : 한국조직공학과 재생의학회
· 수록지 정보 : 조직공학과 재생의학 / 8권 / 1호 / 28 ~ 36페이지
· 저자명 : 서영권, 신연호, 조현우, 박정극, 송계용

초록

To study the correlation of physical properties and degradation rate of cross-linked collagen matrix by chemicals in vivo, non-crosslinked collagen matrix, carbodiimide-crosslinked collagen matrix, and glutaraldehydecrosslinked collagen matrix were implanted in rat subcutaneous tissue. Change of structure was evaluated by scanning electron microscopy, degradation, differential scanning calorimetry, and tensile strength were evaluated in vitro,while inflammatory cell infiltration and degradation degree by light microscopy and transmission-electron microscopy after implantation in vivo. Result of differential scanning calorimeter estimation showed increased the denaturation temperature from 83.87oC to 112.81oC after glutaraldehyde treatment and to 114.56oC after carbodiimide treatment.
And both crosslinked collagen matrixes were more resistant than the non-crosslinked collagen matrix in vitro degradation test with collagenase. The average breaking strength of the non-crosslinked matrix was 0.01 ± 0.005 MPa,whereas strength of the glutaraldehyde-crosslinked matrix was 0.029 ± 0.005 N. After implantation of the matrix in the subcutaneous tissue of rat, more infiltration of neutrophils was noted in glutaraldehyde-crosslinked matrix than in carbodiimide-crosslinked matrix at day 10 and day 21. Major difference was the degradation rate of the matrix,showing that non-crosslinked matrix was degraded about 90% after 21 days, whereas crosslinked matrix remained about 70~80%. This in vivo study revealed that degradation rate of the implanted matrix was much more depend upon the inflammatory reaction of chemicals for crosslink of matrix rather than matrix itself. It is concluded that the difference between in vitro and in vivo physical property and its longevity of matrix in implanted sites was closely related with induced inflammatory reaction to the matrix itself and crosslinking chemicals.

영어초록

To study the correlation of physical properties and degradation rate of cross-linked collagen matrix by chemicals in vivo, non-crosslinked collagen matrix, carbodiimide-crosslinked collagen matrix, and glutaraldehydecrosslinked collagen matrix were implanted in rat subcutaneous tissue. Change of structure was evaluated by scanning electron microscopy, degradation, differential scanning calorimetry, and tensile strength were evaluated in vitro,while inflammatory cell infiltration and degradation degree by light microscopy and transmission-electron microscopy after implantation in vivo. Result of differential scanning calorimeter estimation showed increased the denaturation temperature from 83.87oC to 112.81oC after glutaraldehyde treatment and to 114.56oC after carbodiimide treatment.
And both crosslinked collagen matrixes were more resistant than the non-crosslinked collagen matrix in vitro degradation test with collagenase. The average breaking strength of the non-crosslinked matrix was 0.01 ± 0.005 MPa,whereas strength of the glutaraldehyde-crosslinked matrix was 0.029 ± 0.005 N. After implantation of the matrix in the subcutaneous tissue of rat, more infiltration of neutrophils was noted in glutaraldehyde-crosslinked matrix than in carbodiimide-crosslinked matrix at day 10 and day 21. Major difference was the degradation rate of the matrix,showing that non-crosslinked matrix was degraded about 90% after 21 days, whereas crosslinked matrix remained about 70~80%. This in vivo study revealed that degradation rate of the implanted matrix was much more depend upon the inflammatory reaction of chemicals for crosslink of matrix rather than matrix itself. It is concluded that the difference between in vitro and in vivo physical property and its longevity of matrix in implanted sites was closely related with induced inflammatory reaction to the matrix itself and crosslinking chemicals.

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