난청 동물모델에서 신경성장인자에 의한 청력회복과 나선 신경절재생에 관한 연구 (Effect of Neurotrophic Factors on Hearing Restoration and Spiral Ganglion Regeneration in Deafened Animal Model)

서지정보

· 발행기관 : 한국조직공학과 재생의학회
· 수록지 정보 : 조직공학과 재생의학 / 5권 / 4호 / 849 ~ 854페이지
· 저자명 : 오정훈, 박시내, 최혁기, 최미영, 한민아, 박경호, 여상원

초록

Cochlear prostheses for the sensorineural hearing impairment are dependent on survival and excitability of the auditory nerve. Multiple neurotrophic factors have been shown to have important roles in the survival of auditory neurons and protecting spiral ganglion cells. This study evaluated the efficacy of brain-derived neurotrophic factor(BDNF) combined with glial-cell derived neurotrophic factor(GDNF) and neurotrophin-3(NT-3), insulin growth factor(IGF), and epidermal growth factor(EGF) with fibroblast growth factor(FGF) application in 2 weeks post-deafening guinea pigs. Healthy 30 guinea pigs were deafened by infusion of kanamycin and ethacrynic acid. Myringotomy was done in both ears, gelfoams immersed with neurotrophic factors were placed on the round window membrane while gelfoams immersed with saline were applied to control ear. On 1 week after drug application, hearing test was done and the cochleae were collected in every 2 weeks. And the number of spiral ganglion cells was quantitatively analyzed. Significant hearing restoration was observed in group 1(BDNF, GDNF, NT-3 treated group) & group 2(IGF treated group). The number of spiral ganglion cells of group 1 on 5 week after the treatment, and group 3(EGF, FGF treated group) on 3 week after the treatment were significantly increased them compared with control group. However, no statistically significant regeneration was observed in group 2. These findings demonstrate
that BDNF+GDNF+NT-3 neurotrophic factor application through round window membrane have the potential for regeneration of spiral ganglion cells & hearing restoration.

영어초록

Cochlear prostheses for the sensorineural hearing impairment are dependent on survival and excitability of the auditory nerve. Multiple neurotrophic factors have been shown to have important roles in the survival of auditory neurons and protecting spiral ganglion cells. This study evaluated the efficacy of brain-derived neurotrophic factor(BDNF) combined with glial-cell derived neurotrophic factor(GDNF) and neurotrophin-3(NT-3), insulin growth factor(IGF), and epidermal growth factor(EGF) with fibroblast growth factor(FGF) application in 2 weeks post-deafening guinea pigs. Healthy 30 guinea pigs were deafened by infusion of kanamycin and ethacrynic acid. Myringotomy was done in both ears, gelfoams immersed with neurotrophic factors were placed on the round window membrane while gelfoams immersed with saline were applied to control ear. On 1 week after drug application, hearing test was done and the cochleae were collected in every 2 weeks. And the number of spiral ganglion cells was quantitatively analyzed. Significant hearing restoration was observed in group 1(BDNF, GDNF, NT-3 treated group) & group 2(IGF treated group). The number of spiral ganglion cells of group 1 on 5 week after the treatment, and group 3(EGF, FGF treated group) on 3 week after the treatment were significantly increased them compared with control group. However, no statistically significant regeneration was observed in group 2. These findings demonstrate
that BDNF+GDNF+NT-3 neurotrophic factor application through round window membrane have the potential for regeneration of spiral ganglion cells & hearing restoration.

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