제브라피쉬 근육성장에서의 carnosic acid의 효과 (Effects of Carnosic Acid on Muscle Growth in Zebrafish (Danio rerio))

서지정보

· 발행기관 : 한국어류학회
· 수록지 정보 : 한국어류학회지 / 26권 / 3호 / 171 ~ 178페이지
· 저자명 : 김정환, 진덕희, 김영대, 진형주

초록

로즈마리의 주요 성분인 carnosic acid는 carnosol, rosmarinicacid, ursolic acid 등과 같은 폴리페놀의 한 성분으로 다양한 생리활성 기능이 보고되어 있다. 본 연구에서는 로즈마리 유래 폴리페놀인 carnosic acid가 제브라피쉬 근육성장에 미치는 영향을 근육 내 주사와 사료를 통해서 확인해 보았다. 근육 내 주사 실험을 통해서 CA는 제브라 피쉬의 근육 내 단백질 함량을 증가시키고 중성지방의 함량을 감소시켰다. 또한 조직학적 분석 결과 근섬유의 평균 면적이 커지는 근섬유의 과비대 효과를 나타내었다. 사료 실험 결과근육 내 단백질 및 중성지방의 함량에는 영향을 미치지 않았으며 조직학적 분석 결과 근육 내 주사 실험에서와 마찬가지로 근 섬유의 과비대를 유도하였다.

영어초록

Myogenesis is the formation process of multinucleated myofiber with a contractile capacityfrom muscle satellite cell (MSCs) during life. This process is tightly controlled by several transcriptionfactors such as Pax3 and Pax7 (paired box protein 3 and 7), MEF2C (myocyte enhancer factor 2)and MRFs (myogenic regulatory factors) etc. On the contrary, myostatin (MSTN) is a transforming growthfactor-β superfamily, which functions as a negative regulator of skeletal muscle development andgrowth. Carnosic acid (CA) is a major phenolic component in rosemary (Rosmarinus officinalis) andhave been reported various biological activities such as anticancer, antioxidant, antimicrobial and therapeuticagents for amnesia, dementia, alzheimer’s disease. This study was confirmed to effects of CAon muscle cell line and muscle tissue alteration of zebrafish by intramuscular injection or feeding methods.
10 μM CA showed a non-cytotoxic on myoblast and a complete inhibition effect against myostatinactivity on luciferase assay. In intramuscular injection experiment, the total protein and triglycerideamount of 10 μM/kg of CA injected group increased by 11% and decreased by 13% compared to theseof the no injected group. In histology analysis of muscle tissues by hematoxylin/eosin staining, thenumber of muscle fiber of 10 μM/kg of CA injected group decreased by 29% and fiber area increased40% compared to these of no injected group. In feeding experiment, the total protein and triglycerideamount no significance difference compared to these of the normal feeding group. In histology analysis,the number of muscle fiber of 1% CA fed group decreased by 35% and fiber area increased 56%compared to these of normal fed group. We identified that CA have an effect on hypertrophy of musclefiber in adult zebrafish and the results of this study are considered as the basic data that can revealthe mechanisms of muscle formation via gene and protein level analysis.

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