농법과 계절에 따른 탈질세균의 군집 구조와 탈질율 비교 (A comparison of community structure and denitrifying ratio for denitrifying bacteria dependent on agricultural methods and seasons)

서지정보

· 발행기관 : 한국미생물학회
· 수록지 정보 : 미생물학회지 / 53권 / 1호 / 9 ~ 19페이지
· 저자명 : 윤준범, 박경량

초록

농법에 따른 토양성분과 N2O 발생량, 탈질세균 수, 탈질세균의 군집 구조와 T-RFLP 패턴을 계절별로 조사하였다. 토양성분 분석결과 총 탄소량과 총 유기탄소량은 유기농법에서 각각 1.57%, 1.28%, 무농약 농법은 1.52%, 1.24%, 관행농법은 1.40%, 0.95%로 친환경농법에서 유기 탄소량이 비교적 높게 나타났다. N2O 발생량은 5월과 11월 토양이 높았지만 속도는 8월 토양이 빨랐다. 탈질세균 수는 유기농토양은 평균 1.32 ×104 MPN/g, 무농약 토양은 평균 1.17 × 104 MPN/g, 관행농 토양은 평균 6.29 × 103 MPN/g으로 친환경농법 토양이 관행농법 토양에 비해 탈질세균 수가 많은 것을 확인하였다. 계통수 분석 결과, 전체 10개 Cluster 중 유기농법 토양이 6개의 Cluster에 분포되어 친환경 농법 토양이 다양한 군집을 갖는 것을 확인하였다. T-RFLP 패턴의 PCA profile 분석 결과, 유기농법은 넓은 분포를, 관행농법은 좁은 범위의 분포를 나타내고, 무농약농법은 유기농법과 관행농법의 중간에 분포하는 것으로 확인되었다. 따라서 계절과 농법에 따라 탈질세균의 분포와 군집구조가 달라지는 것을 확인하였다.

영어초록

We studied soil composition, N2O production, a number of denitrifying bacteria, community structure and T-RFLP patterns of denitrifying bacteria dependent on agricultural methods with the change of seasons. Analyses of the soil chemical composition revealed that total carbon and total organic carbon contents were 1.57% and 1.28% in the organic farming soil, 1.52% and 1.24% in the emptiness farming soil, and 1.40% and 0.95% in traditional farming soil, respectively. So, the amount of organic carbon was relatively high in the environment friendly farming soils than traditional farming soils. In case of N2O production, the amount of N2O production was high in May and November soils, but the rate of N2O production was fast in August soil. The average number of denitrifying bacteria were 1.32 × 104 MPN · g-1 in the organic farming soil, 1.17 × 104 MPN · g-1 in the emptiness farming soil, and 6.29 × 103 MPN · g-1 in the traditional farming soil. It was confirmed that the environment friendly farming soil have a larger number of denitrifying bacteria than the traditional farming soil. As a result of the phylogenetic analyses, it was confirmed that six clusters were included in organic farming soil among total 10 clusters. And the result of PCA profile distribution of T-RFLP pattern on agricultural methods, the range of distribution showed wide in the organic farming method, relatively narrow in the conventional farming method, and middle in the emptiness farming method. Therefore, we could concluded that the distribution and the community structure of denitrifying bacteria were changed according to the agricultural methods and seasons.

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