육회와 육사시미에 접종된 Salmonella Typhimurium와 Listeria monocytogenes 검출을 위한 Loop-mediated isothermal amplification와 식품공전의 배지 시험법, real-time PCR의 검출 성능 비교 (Comparison of Loop-mediated Isothermal Amplification and Korea Standard Food Codex (KFSC) Method for Detection of Salmonella Typhimurium, Listeria monocytogenes Artificially Inoculated in Yuk-hwe and )

서지정보

· 발행기관 : 한국식품위생안전성학회
· 수록지 정보 : 한국식품위생안전성학회지 / 34권 / 3호 / 277 ~ 282페이지
· 저자명 : 곽승해, 이소영, 김진희, 오세욱

초록

The object of this study is to compare the performance of the 3M Molecular Detection Assay 2 (3M MDA 2) and the Korea Standard Food Codex (KSFC) Method (i.e., isolation media and real-time PCR) in detect-ing Salmonella Typhimurium and Listeria monocytogenes in traditional Korean foods. Yuk-hwe and Yuk-sashimi (types of raw beef dishes) were artificially inoculated with 100–104 CFU/25 g of L. monocytogenes and S. Typh-imurium. Citrobacter freundii and Listeria innocua were used as competitive microflora. After enrichment, the sam-ples were analyzed using 3M MDA 2 and real-time PCR. All samples inoculated at concentrations of 100–104 CFU/ 25 g without competitive microflora were positive for S. Typhimurium and L. monocytogenes, as detected by 3M MDA 2 and Korea Standard Food Codex (KFSC) Method. In addition, part of the samples were positive for the pres-ence of C. freundii and L. innocua. The 3M MDA 2 – Salmonella and Korea Standard Food Codex (KFSC) Method showed similar detection performances in Yuk-hwe and Yuk-sashimi. The 3M MDA 2 method for Salmonella and Listeria, which is a LAMP-based technology, can be used for rapid detection of S. Typhimurium and L. monocyto-genes in raw beef. LAMP bioluminescence assays provide results on the subsequent day and are simple to use com-pared with the Korea Standard Food Codex (KFSC) Method, particularly in terms of DNA preparation.

영어초록

The object of this study is to compare the performance of the 3M Molecular Detection Assay 2 (3M MDA 2) and the Korea Standard Food Codex (KSFC) Method (i.e., isolation media and real-time PCR) in detect-ing Salmonella Typhimurium and Listeria monocytogenes in traditional Korean foods. Yuk-hwe and Yuk-sashimi (types of raw beef dishes) were artificially inoculated with 100–104 CFU/25 g of L. monocytogenes and S. Typh-imurium. Citrobacter freundii and Listeria innocua were used as competitive microflora. After enrichment, the sam-ples were analyzed using 3M MDA 2 and real-time PCR. All samples inoculated at concentrations of 100–104 CFU/ 25 g without competitive microflora were positive for S. Typhimurium and L. monocytogenes, as detected by 3M MDA 2 and Korea Standard Food Codex (KFSC) Method. In addition, part of the samples were positive for the pres-ence of C. freundii and L. innocua. The 3M MDA 2 – Salmonella and Korea Standard Food Codex (KFSC) Method showed similar detection performances in Yuk-hwe and Yuk-sashimi. The 3M MDA 2 method for Salmonella and Listeria, which is a LAMP-based technology, can be used for rapid detection of S. Typhimurium and L. monocyto-genes in raw beef. LAMP bioluminescence assays provide results on the subsequent day and are simple to use com-pared with the Korea Standard Food Codex (KFSC) Method, particularly in terms of DNA preparation.

참고자료

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