근적외 분광분석법을 이용한 녹차의 색도 분석 (Determination of Color Value (L, a, b) in Green Tea Using Near-Infrared Reflectance Spectroscopy)

서지정보

· 발행기관 : 한국작물학회
· 수록지 정보 : 한국작물학회지 / 53권 / 108 ~ 114페이지
· 저자명 : 이민석, 정명근

초록

Near infrared spectroscopy (NIRS) is a
rapid and accurate analytical method for determining the
composition of agricultural products and feeds. The applicability
of near infrared reflectance spectroscopic method
was tested to determine the color value (L, a, b) of green
tea. A total of 162 green tea calibration samples and 82
validation samples were used for NIRS equation development
and validation, respectively. In the developed NIRS
equation for analysis of the color value (L, a, b), the most
accurate equation for L value was obtained at 2, 8, 6, 1
(2nd derivative, 8 nm gap, 6 points smoothing, and 1point
second smoothing), and for a, and b value were obtained
at 1, 4, 4, 1 (1st derivative, 4 nm gap, 4points smoothing,
and 1 point second smoothing) math treatment condition
with SNVD (Standard Normal Variate and Detrend) scatter
correction method and entire spectrum (400~2,500 nm) by
using MPLS (Modified Partial Least Squares) regression.
Validation results of these NIRS equations showed very
low bias (L: 0.005%, a: 0.003%, b: -0.013%) and standard
error of prediction (SEP, L: 0.361%, a: 0.141%, b: 0.306%)
as well as high coefficient of determination (R2, L: 0.905,
a: 0.986, b: 0.931). Therefore, these NIRS equations can
be applicable and reliable for determination of color value
(L, a, b) of green tea, and NIRS method could be used as
a mass screening technique for breeding programs and
quality control in the green tea industry.

영어초록

Near infrared spectroscopy (NIRS) is a
rapid and accurate analytical method for determining the
composition of agricultural products and feeds. The applicability
of near infrared reflectance spectroscopic method
was tested to determine the color value (L, a, b) of green
tea. A total of 162 green tea calibration samples and 82
validation samples were used for NIRS equation development
and validation, respectively. In the developed NIRS
equation for analysis of the color value (L, a, b), the most
accurate equation for L value was obtained at 2, 8, 6, 1
(2nd derivative, 8 nm gap, 6 points smoothing, and 1point
second smoothing), and for a, and b value were obtained
at 1, 4, 4, 1 (1st derivative, 4 nm gap, 4points smoothing,
and 1 point second smoothing) math treatment condition
with SNVD (Standard Normal Variate and Detrend) scatter
correction method and entire spectrum (400~2,500 nm) by
using MPLS (Modified Partial Least Squares) regression.
Validation results of these NIRS equations showed very
low bias (L: 0.005%, a: 0.003%, b: -0.013%) and standard
error of prediction (SEP, L: 0.361%, a: 0.141%, b: 0.306%)
as well as high coefficient of determination (R2, L: 0.905,
a: 0.986, b: 0.931). Therefore, these NIRS equations can
be applicable and reliable for determination of color value
(L, a, b) of green tea, and NIRS method could be used as
a mass screening technique for breeding programs and
quality control in the green tea industry.

참고자료

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