유즙내에서 메티실린 내성을 지닌 젖소 유방염 주요 원인균에 대한 항생제와 EDTA-Tris의 병합의 효과 (The Effect of Antibiotics in Combination with EDTA-Tris on the Methicillin- Resistant Major Pathogens of Bovine Mastitis in Milk)

서지정보

· 발행기관 : 한국임상수의학회
· 수록지 정보 : 한국임상수의학회지 / 25권 / 5호 / 346 ~ 354페이지
· 저자명 : 유종현, 박희명

초록

The combined effects of EDTA-Tris and eighteen antimicrobial agents have been evaluated in eight clinically
isolated methicillin-resistant bacteria (Staphylococcus aureus, Escherichia coli, Streptococcus uberis and Streptococcus
agalactiae) from bovine mastitis. The antimicrobial activity was evaluated by measuring the minimal bactericidal
concentration (MBC) for the antibiotics alone or in combination with EDTA-Tris in Mueller-Hilton broth and milk.
Combined use of EDTA-Tris and antibiotics potentiated or antagonized activity of antibiotics against mastitic pathogens.
Milk increased the antibiotic potency of erythromycin and spiramycin on S. aureus. Culture in milk changed patterns
of EDTA-Tris combinational effects compared with that in standard Mueller-Hilton broth. Combined with EDTA-Tris
in milk, synergic effects were observed in colistin, dihydrostreptomycin, kanamycin, erythromycin, gentamycin,
oxytetracycline, streptomycin to E. coli, Str. uberis, and Str. agalactiae. However, significant antagonistic effects of
milk on antibiotic susceptibility in combination with EDTA-Tris were noted in neomycin, streptomycin, penicillin,
roxithromycin, and amoxicillin. This study indicates that combination therapy of EDTA-Tris with antibiotics in bovine
mastitis should be used with caution because of the possible antagonistic effects of antibiotic combination with EDTATris
on mastitic pathogens. In addition, antibiotic susceptibility test in combination with EDTA-Tris in milk culture
condition can be benefit in search of effective treatment regimen for some antibiotic-resistant bacteria of mastitis.

영어초록

The combined effects of EDTA-Tris and eighteen antimicrobial agents have been evaluated in eight clinically
isolated methicillin-resistant bacteria (Staphylococcus aureus, Escherichia coli, Streptococcus uberis and Streptococcus
agalactiae) from bovine mastitis. The antimicrobial activity was evaluated by measuring the minimal bactericidal
concentration (MBC) for the antibiotics alone or in combination with EDTA-Tris in Mueller-Hilton broth and milk.
Combined use of EDTA-Tris and antibiotics potentiated or antagonized activity of antibiotics against mastitic pathogens.
Milk increased the antibiotic potency of erythromycin and spiramycin on S. aureus. Culture in milk changed patterns
of EDTA-Tris combinational effects compared with that in standard Mueller-Hilton broth. Combined with EDTA-Tris
in milk, synergic effects were observed in colistin, dihydrostreptomycin, kanamycin, erythromycin, gentamycin,
oxytetracycline, streptomycin to E. coli, Str. uberis, and Str. agalactiae. However, significant antagonistic effects of
milk on antibiotic susceptibility in combination with EDTA-Tris were noted in neomycin, streptomycin, penicillin,
roxithromycin, and amoxicillin. This study indicates that combination therapy of EDTA-Tris with antibiotics in bovine
mastitis should be used with caution because of the possible antagonistic effects of antibiotic combination with EDTATris
on mastitic pathogens. In addition, antibiotic susceptibility test in combination with EDTA-Tris in milk culture
condition can be benefit in search of effective treatment regimen for some antibiotic-resistant bacteria of mastitis.

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