금은화, 연교 및 황련해독탕 약침이 아토피 피부염 유발 NC/Nga 생쥐에 미치는 영향 (The Effects of Lonicerae Flos, Forsythiae Fluctus and Hwangryunhaedok Decoction Pharmacopuncture on Atopic Dermatitis in NC/Nga Mice)

서지정보

· 발행기관 : 대한침구의학회
· 수록지 정보 : Journal of Acupuncture Research / 32권 / 4호 / 119 ~ 131페이지
· 저자명 : 유정석, 김종욱, 이창현, 이상룡, 육태한

초록

Objectives : Atopic dermatitis is a chronic inflammatory skin disease characterized by pruritic and erythematous skin lesions. The purpose of this study was to investigate the suppressive effects of Lonicerae Flos, Forsythiae Fluctus and Hwangryunhaedok Decoction Pharmacop- uncture on the development of atopic dermatitis-like skin lesions in NC/Nga Mice.
Methods: The Atopic Dermatitis was induced by biostir AD on the mice's back skin. Experimental groups were divided into three including LFP(Lonicerae Flos Pharmacopuncture, EtOH extract), FFP(Forsythiae Fluctus Pharmacopuncture, EtOH extract) and HHP(Hwangryunhaedok Decoc- tion Pharmacopuncture, Hydrodistillation extract). Every second day, the mice of three groups were treated with 0.1 ㎖ of pharmacopuncture using a syringe at right and left acupoints (BL13), alternatively. On the control group, normal saline was used instead of pharmacopuncture. Subsequently optical observation with a handscope, a clinical skin score, Tissue(general/im- mune) mast cell, Serum IgE level, Serum histamine level, and Serum lymphokine(IL-2, IL-4, IFN－γγ) were measured.
Results: FFP and HHP decreased the clinical skin score, the total cell number of mast cells, and the Serum total IgE level and Serum histamine level. In Serum lymphokine levels, all groups were decreased to the IL-4 level, LFP and FFP were increased to the IL-2 level, and LFP was increased to the IFN－γlevel.
Conclusions : From the above results, Forsythiae Fluctus Pharmacopuncture (EtOH extract) and Hwangryunhaedok Decoction Pharmacopuncture (Hydrodistillation extract) exerted anti-allergic and anti-inflammatory effects, suggesting a promising agent for improving atopic dermatitis re- lated symptoms.

영어초록

Objectives : Atopic dermatitis is a chronic inflammatory skin disease characterized by pruritic and erythematous skin lesions. The purpose of this study was to investigate the suppressive effects of Lonicerae Flos, Forsythiae Fluctus and Hwangryunhaedok Decoction Pharmacop- uncture on the development of atopic dermatitis-like skin lesions in NC/Nga Mice.
Methods: The Atopic Dermatitis was induced by biostir AD on the mice's back skin. Experimental groups were divided into three including LFP(Lonicerae Flos Pharmacopuncture, EtOH extract), FFP(Forsythiae Fluctus Pharmacopuncture, EtOH extract) and HHP(Hwangryunhaedok Decoc- tion Pharmacopuncture, Hydrodistillation extract). Every second day, the mice of three groups were treated with 0.1 ㎖ of pharmacopuncture using a syringe at right and left acupoints (BL13), alternatively. On the control group, normal saline was used instead of pharmacopuncture. Subsequently optical observation with a handscope, a clinical skin score, Tissue(general/im- mune) mast cell, Serum IgE level, Serum histamine level, and Serum lymphokine(IL-2, IL-4, IFN－γγ) were measured.
Results: FFP and HHP decreased the clinical skin score, the total cell number of mast cells, and the Serum total IgE level and Serum histamine level. In Serum lymphokine levels, all groups were decreased to the IL-4 level, LFP and FFP were increased to the IL-2 level, and LFP was increased to the IFN－γlevel.
Conclusions : From the above results, Forsythiae Fluctus Pharmacopuncture (EtOH extract) and Hwangryunhaedok Decoction Pharmacopuncture (Hydrodistillation extract) exerted anti-allergic and anti-inflammatory effects, suggesting a promising agent for improving atopic dermatitis re- lated symptoms.

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