H2O2 유도 U2OS 세포 및 골관절염 동물 모델에 대한 Cotoneaster mongolicus의 효과 (Cotoneaster mongolicus Effects on H2O2-Induced U2OS Cells and Animal Models of Osteoarthritis)

서지정보

· 발행기관 : 대한본초학회
· 수록지 정보 : 대한본초학회지 / 39권 / 6호 / 33 ~ 43페이지
· 저자명 : 김건우, 김민주, 신미래, 안희연, 노성수

초록

Objectives：Cotoneaster mongolicus, one of the plants in the genus Cotoneaster, is known to have anti-inflammatory and antioxidant effects. In this study, we investigated the effects of Cotoneaster mongolicus extract (CME) on joint health.
Methods：To evaluate the effects of CME on U2OS cells, an osteosarcoma cell line, the cells were pretreated with 200 μg/mL of CME for 4 h before being exposed to 500 μM H2O2 for overnight incubation. Following this, MitoSox, MitoTracker, 4-HNE, and ColT2 staining were performed. For in vivo experiments, MIA was injected into the right joint cavity of SD rats to induce osteoarthritis. One week later, indomethacin (2 mg/kg) or CME (200 mg/kg) was administered orally for 2 weeks. After the treatment period, the levels of pro-inflammatory cytokines in serum, as well as inflammation-related proteins and MMPs in the knee joint, were assessed.
Results：As a result, CME treatment effectively mitigated oxidative stress induced by H2O2 and improved damaged mitochondria. Additionally, immunohistochemical staining demonstrated that CME decreased the levels of 4-HNE elevated by H2O2 and restored the reduced levels of ColT2. Furthermore, CME significantly inhibited the expression of MMPs, enzymes responsible for degrading the extracellular matrix, by modulating the levels of pro-inflammatory cytokines in serum and regulating the NF-κBp65 signaling pathway in the knee joint.
Conclusion：In summary, our results demonstrate that CME beneficial effects on joint health by reducing oxidative stress in H2O2-treated U2OS cells and attenuating knee joint injury in an osteoarthritis animal model.

영어초록

Objectives：Cotoneaster mongolicus, one of the plants in the genus Cotoneaster, is known to have anti-inflammatory and antioxidant effects. In this study, we investigated the effects of Cotoneaster mongolicus extract (CME) on joint health.
Methods：To evaluate the effects of CME on U2OS cells, an osteosarcoma cell line, the cells were pretreated with 200 μg/mL of CME for 4 h before being exposed to 500 μM H2O2 for overnight incubation. Following this, MitoSox, MitoTracker, 4-HNE, and ColT2 staining were performed. For in vivo experiments, MIA was injected into the right joint cavity of SD rats to induce osteoarthritis. One week later, indomethacin (2 mg/kg) or CME (200 mg/kg) was administered orally for 2 weeks. After the treatment period, the levels of pro-inflammatory cytokines in serum, as well as inflammation-related proteins and MMPs in the knee joint, were assessed.
Results：As a result, CME treatment effectively mitigated oxidative stress induced by H2O2 and improved damaged mitochondria. Additionally, immunohistochemical staining demonstrated that CME decreased the levels of 4-HNE elevated by H2O2 and restored the reduced levels of ColT2. Furthermore, CME significantly inhibited the expression of MMPs, enzymes responsible for degrading the extracellular matrix, by modulating the levels of pro-inflammatory cytokines in serum and regulating the NF-κBp65 signaling pathway in the knee joint.
Conclusion：In summary, our results demonstrate that CME beneficial effects on joint health by reducing oxidative stress in H2O2-treated U2OS cells and attenuating knee joint injury in an osteoarthritis animal model.

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